論文

査読有り
2008年2月

New primary culture systems to study the differentiation and proliferation of mouse fetal hepatoblasts

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
  • Rika Miki
  • ,
  • Norifumi Tatsumi
  • ,
  • Ken Matsumoto
  • ,
  • Yuji Yokouchi

294
2
開始ページ
G529
終了ページ
G539
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1152/ajpgi.00412.2007
出版者・発行元
AMER PHYSIOLOGICAL SOC

Hepatoblasts have the potential to differentiate into both hepatocytes and biliary epithelial cells through a differentiation program that has not been fully elucidated. With the aim to better define the mechanism of differentiation of hepatoblasts, we isolated hepatoblasts and established new culture systems. We isolated hepatoblasts from E12.5 fetal mouse liver by using E-cadherin. The E-cadherin + cells expressed alpha- fetoprotein (AFP) and albumin (Alb) but not cytokeratin 19 (CK19). Transplantation of the E-cadherin + cells into mice that had been subjected to liver injury or biliary epithelial injury led to differentiation of the cells into hepatocytes or biliary epithelial cells, respectively. In a low-cell-density culture system in the absence of additional growth factors, E-cadherin + cells formed colonies of various sizes, largely comprising Alb-positive cells. Supplementation of the culture medium with hepatocyte growth factor and epidermal growth factor promoted proliferation of the cells. Thus the low-cell-density culture system should be useful to identify inductive factors that regulate the proliferation and differentiation of hepatoblasts. In a high-cell-density system in the presence of oncostatin M + dexamethasone, E14.5, but not E12.5, E-cadherin + cells differentiated into mature hepatocytes, suggesting that unidentified factors are involved in hepatic maturation. Culture of E-cadherin + cells derived from E12.5 or E14.5 liver under high-celldensity conditions should allow elucidation of the mechanism of hepatic differentiation in greater detail. These new culture systems should be of use to identify growth factors that induce hepatoblasts to proliferate or differentiate into hepatocytes and biliary epithelial cells.

リンク情報
DOI
https://doi.org/10.1152/ajpgi.00412.2007
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18096607
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000253120100019&DestApp=WOS_CPL
ID情報
  • DOI : 10.1152/ajpgi.00412.2007
  • ISSN : 0193-1857
  • PubMed ID : 18096607
  • Web of Science ID : WOS:000253120100019

エクスポート
BibTeX RIS