Papers

Peer-reviewed
Sep 1, 2017

Th9 cells induce steroid-resistant bronchial hyperresponsiveness in mice

Allergology International
  • Mayumi Saeki
  • ,
  • Osamu Kaminuma
  • ,
  • Tomoe Nishimura
  • ,
  • Noriko Kitamura
  • ,
  • Akio Mori
  • ,
  • Takachika Hiroi

Volume
66
Number
S
First page
S35
Last page
S40
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.alit.2017.07.001
Publisher
Japanese Society of Allergology

Background Reduced responsiveness to corticosteroid therapy is a major problem for patients with severe asthma. Although Th9 cells, along with Th2 cells, facilitate antigen-induced airway eosinophilia and bronchial hyperresponsiveness (BHR), the sensitivity of Th9 cell-mediated responses to steroid therapy remains unknown. In this study, we investigated the effect of dexamethasone (Dex) on antigen-induced airway inflammation in Th9 cell-transferred mice. Methods Ovalbumin (OVA)-specific Th2 and Th9 cells were polarized from the CD4+ T cells of DO11.10/RAG-2−/− mice. BALB/c mice were adoptively transferred with Th2 or Th9 cells and challenged with OVA. Dex treatment was performed twice, at 1 h before and at 24 h after the OVA challenge. Following treatment, the number of inflammatory cells in the bronchoalveolar lavage fluid and the bronchial responsiveness to inhaled methacholine were determined. Results In both the Th2 and Th9 cell-transferred mice, substantial accumulation of eosinophils in the lungs and BHR were induced by challenge with the specific antigen. In the Th2 cell-transferred mice, these responses were significantly diminished by Dex treatment. In contrast, neither cellular infiltration nor BHR was affected by Dex treatment in the Th9 cell-transferred mice, although the Th9 cells substantially expressed glucocorticoid receptor α. Accordingly, antigen-induced interleukin-9 expression in the Th9 cells was attenuated by Dex treatment at least in vitro. Antigen-induced lung infiltration of infused Th2 cells but not Th9 cells was significantly suppressed by Dex. Conclusions In contrast to Th2-mediated responses, Th9-mediated airway inflammation was not affected by Dex. Th9 cells might be involved in the developmental mechanisms of steroid-resistant asthma.

Link information
DOI
https://doi.org/10.1016/j.alit.2017.07.001
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28755856
ID information
  • DOI : 10.1016/j.alit.2017.07.001
  • ISSN : 1440-1592
  • ISSN : 1323-8930
  • Pubmed ID : 28755856
  • SCOPUS ID : 85025813048

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