1993年12月
CDNA TRANSFECTION FOLLOWED BY THE ISOLATION OF A MCF-7 BREAST CELL-LINE RESISTANT TO TAMOXIFEN IN-VITRO AND IN-VIVO
BRITISH JOURNAL OF CANCER
- ,
- ,
- 巻
- 68
- 号
- 6
- 開始ページ
- 1088
- 終了ページ
- 1096
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/bjc.1993.486
- 出版者・発行元
- STOCKTON PRESS
A tamoxifen resistant cell line (clone 9) has been isolated from the tamoxifen sensitive, hormone responsive MCF-7 breast carcinoma cell line after transfection with mixed cDNA libraries, followed by tamoxifen selection in the presence of oestrogens. Transfection was confirmed by Southern analysis with vector probes. Clone 9 in several-fold more resistant to tamoxifen and other anti-oestrogens than wild type cells when cultured either as a monolayer or as colonies in soft agar but retains oestrogen receptors. Clone 9 was less responsive to 17-beta-oestradiol than were wild type MCF-7. In addition to showing in vitro tamoxifen resistance, clone 9 was also tamoxifen resistant in vivo when xenografted into the nude mouse. Culture medium conditioned by clone 9 cells stimulated quiescent cells of the same clone as well as wild type cells, whereas medium conditioned by wild type MCF-7 was inhibitory to both, suggesting that clone 9 may be secreting an autocrine growth factor. Clone 9 provides a novel model for further investigation of the mechanism of anti-oestrogen resistance that occurs without loss of oestrogen receptors. Preliminary results suggest that an autocrine growth stimulatory mechanism may be one pathway of such resistance.
- リンク情報
- ID情報
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- DOI : 10.1038/bjc.1993.486
- ISSN : 0007-0920
- Web of Science ID : WOS:A1993MK53100006