論文

査読有り
2004年2月

Quantification of vascular endothelial growth factor-C (VEGF-C) by a novel ELISA

JOURNAL OF IMMUNOLOGICAL METHODS
  • HA Weich
  • ,
  • H Bando
  • ,
  • M Brokelmann
  • ,
  • P Baumann
  • ,
  • M Toi
  • ,
  • B Barleon
  • ,
  • K Alitalo
  • ,
  • B Sipos
  • ,
  • J Sleeman

285
2
開始ページ
145
終了ページ
155
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jim.2003.10.015
出版者・発行元
ELSEVIER SCIENCE BV

Lymphangiogenesis plays an important role in several normal and pathological conditions such as wound healing, inflammation or metastasis formation in several malignancies. VEGF-C and VEGF-D are important and specific regulatory factors for lymphatic endothelial proliferation and lymphangiogenesis. In order to develop a highly sensitive and specific detection system for VEGF-C, we produced soluble binding proteins and antibodies for a microtiterplate-based assay. Here we describe a specific enzyme-linked immunosorbent assay (ELISA) for the measurement of human, rat and murine VEGF-C. The different antibodies developed against human and rat VEGF-C could be combined to detect processed and partially processed VEGF-C in a specific way. The ELISA was able to detect human and rat VEGF-C with a minimum detection limit of 100 pg/ml. The assay did not show any cross-reactivity with the related protein VEGF-D. Furthermore, complex formation with its soluble receptors VEGFR-2 and VEGFR-3 did not restricted the sensitivity of the assay. Using this assay, VEGF-C was measured in supernatants and lysates of different cell types and in turnout tissue samples of murine, rat and human origin. Cell lines secrete VEGF-C in very low amounts (<1 ng/ml) whereas VEGF-C transfected cells can secrete up to 50 ng/ml VEGF-C into the supernatant. In human tumour tissue samples VEGF-C was detected in some carcinomas in the low protein range. This ELISA will be a useful tool for investigations concerning the physiological function of VEGF-C in lymphangiogenesis under normal and pathophysiological conditions. (C) 2004 Elsevier B.V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.jim.2003.10.015
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000220140200001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.jim.2003.10.015
  • ISSN : 0022-1759
  • Web of Science ID : WOS:000220140200001

エクスポート
BibTeX RIS