2009年3月
Identification of the gene encoding pro-phenoloxidase A(3) in the fruitfly, Drosophila melanogaster
INSECT MOLECULAR BIOLOGY
- ,
- 巻
- 18
- 号
- 2
- 開始ページ
- 223
- 終了ページ
- 232
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1365-2583.2008.00858.x
- 出版者・発行元
- WILEY-BLACKWELL
The fruitfly, Drosophila melanogaster, has three proPO genes (DoxA1, CG8193 and CG2952). DoxA1 has been shown to encode proPO A(1), one of the two proPO isoforms (A(1) and A(3)). However, which of CG8193 or CG2952 encodes proPO A(3) has so far remained elusive. In Northern analysis, CG8193 expression was strong during the larval stage, yet expression of CG2952 was not detected at any stage. Immunoblot analyses with specific antibodies detected CG8193 in the larval hemolymph at the mobility of the endogenous proPO A(3), though no signal for CG2952. These results indicate that the expression of CG2952 is very low and that CG8193 is the gene that encodes proPO A(3). Processing of A(1) and A(3) isoforms in adult homogenate and activity of recombinant proPOs were also investigated.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1365-2583.2008.00858.x
- ISSN : 0962-1075
- Web of Science ID : WOS:000264185800010