論文

査読有り
2016年6月

Colletotrichum orbiculare WHI2, a Yeast Stress-Response Regulator Homolog, Controls the Biotrophic Stage of Hemibiotrophic Infection Through TOR Signaling

MOLECULAR PLANT-MICROBE INTERACTIONS
  • Ken Harata
  • ,
  • Takumi Nishiuchi
  • ,
  • Yasuyuki Kubo

29
6
開始ページ
468
終了ページ
483
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1094/MPMI-02-16-0030-R
出版者・発行元
AMER PHYTOPATHOLOGICAL SOC

The hemibiotrophic fungus Colletotrichum orbiculare first establishes a biotrophic infection stage in cucumber (Cucumber sativus) epidermal cells and subsequently transitions to a necrotrophic stage. Here, we found that C. orbiculare established hemibiotrophic infection via C. orbiculare WHI2, a yeast stress regulator homolog, and TOR (target of rapamycin) signaling. Plant defense responses such as callose deposition, H2O2, and antimicrobial proteins were strongly induced by the C. orbiculare whi2 Delta mutant, resulting in defective pathogenesis. Expression analysis of biotrophy-specific genes evaluated by the promoter VENUS fusion gene indicated weaker VENUS signal intensity in the whi2 Delta mutant, thereby suggesting that C. orbiculare WHI2 plays a key role in regulating biotrophic infection of C. orbiculare. The involvement of CoWHI2 in biotrophic infection was further explored with a DNA microarray. In the Cowhi2 Delta. mutant, TOR-dependent ribosomal protein related genes were strikingly upregulated compared with the wild type. Moreover, callose deposition in the host plant after inoculation with the Cowhi2 Delta mutant treated with rapamycin, which inhibits TOR activity, was reduced, and the mutant remained biotrophic in contrast to the untreated mutant. Thus, regulation of TOR by Whi2 is apparently crucial to the biotrophic stage of hemibiotrophic infection in C. orbiculare.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1094/MPMI-02-16-0030-R
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27018615
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000376813500004&DestApp=WOS_CPL

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