論文

査読有り 最終著者 責任著者 国際誌
2021年

Metabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.

PloS one
  • Haruya Takahashi
  • Koji Ochiai
  • Kuni Sasaki
  • Atsushi Izumi
  • Yu Shinyama
  • Shinsuke Mohri
  • Wataru Nomura
  • Huei-Fen Jheng
  • Teruo Kawada
  • Kazuo Inoue
  • Tsuyoshi Goto
  • 全て表示

16
7
開始ページ
e0254190
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0254190

Several isoflavonoids are well known for their ability to act as soybean phytoalexins. However, the overall effects of the soybean-Aspergillus oryzae interaction on metabolism remain largely unknown. The aim of this study is to reveal an overview of nutritive and metabolic changes in germinated and A. oryzae-elicited soybeans. The levels of individual nutrients were measured using the ustulation, ashing, Kjeldahl, and Folch methods. The levels of individual amino acids were measured using high-performance liquid chromatography. Low-molecular-weight compounds were measured through metabolome analysis using liquid chromatography-mass spectrometry. Although the levels of individual nutrients and amino acids were strongly influenced by the germination process, the elicitation process had little effect on the change in the contents of individual nutrients and amino acids. However, after analyzing approximately 700 metabolites using metabolome analysis, we found that the levels of many of the metabolites were strongly influenced by soybean-A. oryzae interactions. In particular, the data indicate that steroid, terpenoid, phenylpropanoid, flavonoid, and fatty acid metabolism were influenced by the elicitation process. Furthermore, we demonstrated that not the germination process but the elicitation process induced daidzein prenylation, suggesting that the soybean-A. oryzae interactions produce various phytoalexins that are valuable for health promotion and/or disease prevention.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0254190
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34214105
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253397
ID情報
  • DOI : 10.1371/journal.pone.0254190
  • PubMed ID : 34214105
  • PubMed Central 記事ID : PMC8253397

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