2015年4月
Effect of Dietary Vitamin E Supplementation on Liver Oxidative Damage in Rats with Water-Immersion Restraint Stress
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
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- 巻
- 61
- 号
- 2
- 開始ページ
- 113
- 終了ページ
- 122
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3177/jnsv.61.113
- 出版者・発行元
- CENTER ACADEMIC PUBL JAPAN
We examined how dietary supplementation of vitamin E protects against liver oxidative damage in rats with water-immersion restraint stress (WIRS). Before WIRS exposure, rats received a normal diet (ND) or vitamin E-supplemented diet (VESD) (500 IU alpha-tocopherol/kg diet) at a mean dose of 15 g/animal/d for 4 wk. The two diet groups had serum transaminases and lactate dehydrogenase activities and adrenocorticotropic hormone, corticosterone, and glucose levels to a similar extent. VESD-fed rats had higher liver a-tocopherol concentrations and lower liver ascorbic acid, total coenzyme Q(9) (CoQ(9)), reduced CoQ(9), reduced CoQ(10), and lipid peroxide (LPO) concentrations than ND-fed rats. When the two diet groups were exposed to 6 h of WIRS, the serum liver cell damage index enzyme activities increased more greatly in ND-fed rats than in VESD-fed rats but the serum stress marker levels increased to a similar extent. The WIRS exposure caused no change in liver LPO concentration with the further increase in liver a-tocopherol concentration in VESD-fed rats but increased liver LPO concentration without changing liver alpha-tocopherol concentration in ND-fed rats. Upon the WIRS exposure, liver reduced glutathione concentration decreased with the further decrease in liver ascorbic acid concentration in VESD-fed rats and those concentrations decreased in ND-fed rats. The WIRS exposure recovered the decreased liver total CoQ(9) and reduced CoQ(9) concentrations in VESD-fed rats but decreased liver total CoQ(9), reduced CoQ(9), and reduced CoQ(10) concentrations in ND-fed rats. These results indicate that dietary vitamin E supplementation protects against liver oxidative damage without affecting the stress response in rats with WIRS.
- リンク情報
- ID情報
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- DOI : 10.3177/jnsv.61.113
- ISSN : 0301-4800
- eISSN : 1881-7742
- PubMed ID : 26052141
- Web of Science ID : WOS:000355020000002