2020年4月
Genome sequence and analysis of a Japanese radish (Raphanus sativus) cultivar named 'Sakurajima Daikon' possessing giant root
DNA RESEARCH
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- 巻
- 27
- 号
- 2
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/dnares/dsaa010
- 出版者・発行元
- OXFORD UNIV PRESS
Aim: The complex genome of a Japanese radish (Raphanus sativus) cultivar named 'Okute-Sakurajima' with an extremely large edible round root was analysed to explore its genomic characteristics.Methods and Results: Single-molecule real-time technology was used to obtain long sequence reads to cover 60x of the genome. De novo assembly generated 504.5Mb contigs consisting of 1,437 sequences with the N50 value of 1.2Mb and included 94.1% of the core eukaryotic genes. Nine pseudomolecules, comprising 69.3% of the assembled contigs, were generated along with a high-density SNP genetic map. The sequence data thus established revealed the presence of structural variations and rearrangements in the Brassicaceae genomes.Conclusion and perspective: A total of 89,915 genes were identified in the 'Okute-Sakurajima' genome, 30,033 of which were newly found in this study. The genome information reported here will not only contribute to the establishment of a new resource for the radish genomics but also provide insights into the molecular mechanisms underlying formation of the giant root.
- リンク情報
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- DOI
- https://doi.org/10.1093/dnares/dsaa010
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/32426809
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334891
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000577168900003&DestApp=WOS_CPL
- URL
- http://academic.oup.com/dnaresearch/advance-article-pdf/doi/10.1093/dnares/dsaa010/33236709/dsaa010.pdf
- ID情報
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- DOI : 10.1093/dnares/dsaa010
- ISSN : 1340-2838
- eISSN : 1756-1663
- ORCIDのPut Code : 76743914
- PubMed ID : 32426809
- PubMed Central 記事ID : PMC7334891
- Web of Science ID : WOS:000577168900003