論文

査読有り 筆頭著者 責任著者
2016年5月

A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
  • Shota Katayama (Corresponding author)
  • ,
  • Tetsuo Moriguchi
  • ,
  • Naoki Ohtsu
  • ,
  • Toru Kondo

55
22
開始ページ
6452
終了ページ
6456
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/anie.201601708
出版者・発行元
WILEY-V C H VERLAG GMBH

Targeted transcriptional activation of endogenous genes is important for understanding physiological transcriptional networks, synthesizing genetic circuits, and inducing cellular phenotype changes. The CRISPR/Cas9 system has great potential to achieve this purpose, however, it has not yet been successfully used to efficiently activate endogenous genes and induce changes in cellular phenotype. A powerful method for transcriptional activation by using CRISPR/Cas9 was developed. Replacement of a methylated promoter with an unmethylated one by CRISPR/Cas9 was sufficient to activate the expression of the neural cell gene OLIG2 and the embryonic stem cell gene NANOG in HEK293T cells. Moreover, CRISPR/Cas9-based OLIG2 activation induced the embryonic carcinoma cell line NTERA-2 to express the neuronal marker beta III-tubulin.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1002/anie.201601708
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27079176
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000377921200015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/anie.201601708
  • ISSN : 1433-7851
  • eISSN : 1521-3773
  • PubMed ID : 27079176
  • Web of Science ID : WOS:000377921200015

エクスポート
BibTeX RIS