May, 2016
A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
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- ,
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- Volume
- 55
- Number
- 22
- First page
- 6452
- Last page
- 6456
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1002/anie.201601708
- Publisher
- WILEY-V C H VERLAG GMBH
Targeted transcriptional activation of endogenous genes is important for understanding physiological transcriptional networks, synthesizing genetic circuits, and inducing cellular phenotype changes. The CRISPR/Cas9 system has great potential to achieve this purpose, however, it has not yet been successfully used to efficiently activate endogenous genes and induce changes in cellular phenotype. A powerful method for transcriptional activation by using CRISPR/Cas9 was developed. Replacement of a methylated promoter with an unmethylated one by CRISPR/Cas9 was sufficient to activate the expression of the neural cell gene OLIG2 and the embryonic stem cell gene NANOG in HEK293T cells. Moreover, CRISPR/Cas9-based OLIG2 activation induced the embryonic carcinoma cell line NTERA-2 to express the neuronal marker beta III-tubulin.
- Link information
- ID information
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- DOI : 10.1002/anie.201601708
- ISSN : 1433-7851
- eISSN : 1521-3773
- Pubmed ID : 27079176
- Web of Science ID : WOS:000377921200015