講演・口頭発表等

2020年11月27日

De Novo Genome Assembly of the Japanese Wheat Cultivar Norin 61 Highlights Functional Variation in Flowering Time and Fusarium Resistance Genes in East Asian Genotypes

Plant and Cell Physiology
  • Kentaro K Shimizu
  • ,
  • Dario Copetti
  • ,
  • Moeko Okada
  • ,
  • Thomas Wicker
  • ,
  • Toshiaki Tameshige
  • ,
  • Masaomi Hatakeyama
  • ,
  • Rie Shimizu-Inatsugi
  • ,
  • Catharine Aquino
  • ,
  • Kazusa Nishimura
  • ,
  • Fuminori Kobayashi
  • ,
  • Kazuki Murata
  • ,
  • Tony Kuo
  • ,
  • Emily Delorean
  • ,
  • Jesse Poland
  • ,
  • Georg Haberer
  • ,
  • Manuel Spannagl
  • ,
  • Klaus F X Mayer
  • ,
  • Juan Gutierrez-Gonzalez
  • ,
  • Gary J Muehlbauer
  • ,
  • Cecile Monat
  • ,
  • Axel Himmelbach
  • ,
  • Sudharsan Padmarasu
  • ,
  • Martin Mascher
  • ,
  • Sean Walkowiak
  • ,
  • Tetsuya Nakazaki
  • ,
  • Tomohiro Ban
  • ,
  • Kanako Kawaura
  • ,
  • Hiroyuki Tsuji
  • ,
  • Curtis Pozniak
  • ,
  • Nils Stein
  • ,
  • Jun Sese
  • ,
  • Shuhei Nasuda
  • ,
  • Hirokazu Handa

開催年月日
2020年11月27日 - 2020年11月27日
記述言語
英語
会議種別
主催者
Oxford University Press (OUP)

<title>Abstract</title>
Bread wheat is a major crop that has long been the focus of basic and breeding research. Assembly of its genome has been difficult because of its large size and allohexaploid nature (AABBDD genome). Following the first reported assembly of the genome of the experimental strain Chinese Spring (CS), the 10+ Wheat Genomes Project was launched to produce multiple assemblies of worldwide modern cultivars. The only Asian cultivar in the project is Norin 61, a representative Japanese cultivar adapted to grow across a broad latitudinal range, mostly characterized by a wet climate and a short growing season. Here, we characterize key aspects of its chromosome-scale genome assembly spanning 15 Gb with a raw scaffold N50 of 23 Mb. Analysis of the repetitive elements identified chromosomal regions unique to Norin 61 that encompass a tandem array of the pathogenesis-related-13 family. We report novel copy-number variations in the B homeolog of the florigen gene FT1/VRN3, pseudogenization of its D homeolog, and the association of its A homeologous alleles with the spring/winter growth habit. Further, the Norin 61 genome carries typical East Asian functional variants from CS ranging from a single nucleotide to multi-Mb scale. Examples of such variation are the Fhb1 locus, which confers Fusarium head-blight resistance, Ppd-D1a, which confers early flowering, Glu-D1f for Asian noodle quality, and Rht-D1b, which introduced semi-dwarfism during the green revolution. The adoption of Norin 61 as a reference assembly for functional and evolutionary studies will enable comprehensive characterization of the underexploited Asian bread wheat diversity.

リンク情報
共同研究・競争的資金等の研究課題
倍数体マルチオミクス技術開発による環境頑健性付与モデルの構築
URL
http://academic.oup.com/pcp/advance-article-pdf/doi/10.1093/pcp/pcaa152/34551179/pcaa152.pdf
DOI
https://doi.org/10.1093/pcp/pcaa152