論文

査読有り
2014年11月

MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane

NATURE COMMUNICATIONS
  • Yoshinori Sato
  • ,
  • Kenji Hayashi
  • ,
  • Yoshiko Amano
  • ,
  • Mikiko Takahashi
  • ,
  • Shigenobu Yonemura
  • ,
  • Ikuko Hayashi
  • ,
  • Hiroko Hirose
  • ,
  • Shigeo Ohno
  • ,
  • Atsushi ' Suzuki

5
開始ページ
5266
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/ncomms6266
出版者・発行元
NATURE PUBLISHING GROUP

Recent studies have revealed the presence of a microtubule subpopulation called Golgi-derived microtubules that support Golgi ribbon formation, which is required for maintaining polarized cell migration. CLASPs and AKAP450/CG-NAP are involved in their formation, but the underlying molecular mechanisms remain unclear. Here, we find that the microtubule-crosslinking protein, MTCL1, is recruited to the Golgi membranes through interactions with CLASPs and AKAP450/CG-NAP, and promotes microtubule growth from the Golgi membrane. Correspondingly, MTCL1 knockdown specifically impairs the formation of the stable perinuclear microtubule network to which the Golgi ribbon tethers and extends. Rescue experiments demonstrate that besides its crosslinking activity mediated by the N-terminal microtubule-binding region, the C-terminal microtubule-binding region plays essential roles in these MTCL1 functions through a novel microtubule-stabilizing activity. These results suggest that MTCL1 cooperates with CLASPs and AKAP450/CG-NAP in the formation of the Golgi-derived microtubules, and mediates their development into a stable microtubule network.

Web of Science ® 被引用回数 : 21

リンク情報
DOI
https://doi.org/10.1038/ncomms6266
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000345685600001&DestApp=WOS_CPL