論文

査読有り
2014年8月

CLASPs Are Required for Proper Microtubule Localization of End-Binding Proteins

DEVELOPMENTAL CELL
  • Ashley D. Grimaldi
  • ,
  • Takahisa Maki
  • ,
  • Benjamin P. Fitton
  • ,
  • Daniel Roth
  • ,
  • Dmitry Yampolsky
  • ,
  • Michael W. Davidson
  • ,
  • Tatyana Svitkina
  • ,
  • Anne Straube
  • ,
  • Ikuko Hayashi
  • ,
  • Irina Kaverina

30
3
開始ページ
343
終了ページ
352
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.devcel.2014.06.026
出版者・発行元
CELL PRESS

Microtubule (MT) plus-end tracking proteins (+TIPs) preferentially localize to MT plus ends. End-binding proteins (EBs) are master regulators of the +TIP complex; however, it is unknown whether EBs are regulated by other +TIPs. Here, we show that cytoplasmic linker-associated proteins (CLASPs) modulate EB localization at MTs. In CLASP-depleted cells, EBs localized along the MT lattice in addition to plus ends. The MT-binding region of CLASP was sufficient for restoring normal EB localization, whereas neither EB-CLASP interactions nor EB tail-binding proteins are involved. In vitro assays revealed that CLASP directly functions to remove EB from MTs. Importantly, this effect occurs specifically during MT polymerization, but not at preformed MTs. Increased GTP-tubulin content within MTs in CLASP-depleted cells suggests that CLASPs facilitate GTP hydrolysis to reduce EB lattice binding. Together, these findings suggest that CLASPs influence the MT lattice itself to regulate EB and determine exclusive plus-end localization of EBs in cells.

Web of Science ® 被引用回数 : 19

リンク情報
DOI
https://doi.org/10.1016/j.devcel.2014.06.026
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000340344600011&DestApp=WOS_CPL