論文

査読有り
2016年

Proliferation-Related Activity in Endothelial Cells Is Enhanced by Micropower Plasma

BIOMED RESEARCH INTERNATIONAL
  • Kotaro Suzuki
  • ,
  • Daisuke Yoshino

2016
開始ページ
4651265
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1155/2016/4651265
出版者・発行元
HINDAWI PUBLISHING CORP

Nonthermal plasma has received a lot of attention as a medical treatment technique in recent years. It can easily create various reactive chemical species (ROS) and is harmless to living body. Although plasma at gas-liquid interface has a potential for a biomedical application, the interactions between the gas-liquid plasma and living cells remain unclear. Here, we show characteristics of a micropower plasma with 0.018 W of the power input, generated at gas-liquid interface. We also provide the evidence of plasma-induced enhancement in proliferation activity of endothelial cells. The plasma produced H2O2, HNO2, and HNO3 in phosphate buffered saline containing Mg++ and Ca++ (PBS(+)), and their concentration increased linearly during 600-second discharge. The value of pH in PBS(+) against the plasma discharge time was stable at about 7.0. Temperature in PBS(+) rose monotonically, and its rise was up to 0.8 degrees C at the bottom of a cell-cultured dish by the plasma discharge for 600s. Short-time treatment of the plasma enhanced proliferation activity of endothelial cells. In contrast, the treatment of H2O2 does not enhance the cell proliferation. Thus, the ROS production and the nuclear factor-kappa B (NF-kappa B) activation due to the plasma treatment might be related to enhancement of the cell proliferation. Our results may potentially provide the basis for developing the biomedical applications using the gas-liquid plasma.

リンク情報
DOI
https://doi.org/10.1155/2016/4651265
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000390531300001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1155/2016/4651265
  • ISSN : 2314-6133
  • eISSN : 2314-6141
  • Web of Science ID : WOS:000390531300001

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