論文

査読有り
2011年8月

Reconstruction of human elastic cartilage by a CD44(+) CD90(+) stem cell in the ear perichondrium

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Shinji Kobayashi
  • ,
  • Takanori Takebe
  • ,
  • Midori Inui
  • ,
  • Sayaka Iwai
  • ,
  • Hiroomi Kan
  • ,
  • Yun-Wen Zheng
  • ,
  • Jiro Maegawa
  • ,
  • Hideki Taniguchi

108
35
開始ページ
14479
終了ページ
14484
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.1109767108
出版者・発行元
NATL ACAD SCIENCES

Despite the great demands for treating craniofacial injuries or abnormalities, effective treatments are currently lacking. One promising approach involves human elastic cartilage reconstruction using autologous stem/progenitor populations. Nevertheless, definitive evidence of the presence of stem cells in human auricular cartilage remains to be established. Here, we demonstrate that human auricular perichondrium, which can be obtained via a minimally invasive approach, harbors a unique cell population, termed as cartilage stem/progenitor cells (CSPCs). The clonogenic progeny of a single CD44(+) CD90(+) CSPC displays a number of features characteristic of stem cells. Highly chondrogenic CSPCs were shown to reconstruct large (>2 cm) elastic cartilage after extended expansion and differentiation. CSPC-derived cartilage was encapsulated by a perichondrium layer, which contains a CD44(+) CD90(+) self-renewing stem/progenitor population and was maintained without calcification or tumor formation even after 10 mo. This is a unique report demonstrating the presence of stem cells in auricular cartilage. Utilization of CSPCs will provide a promising reconstructive material for treating craniofacial defects with successful long-term tissue restoration.

リンク情報
DOI
https://doi.org/10.1073/pnas.1109767108
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21836053
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000294425900028&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.1109767108
  • ISSN : 0027-8424
  • PubMed ID : 21836053
  • Web of Science ID : WOS:000294425900028

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