論文

査読有り
2017年6月

Fluorescence property of photosystem II protein complexes bound to a gold nanoparticle

FARADAY DISCUSSIONS
  • Kazuki Tahara
  • ,
  • Ahmed Mohamed
  • ,
  • Kousuke Kawahara
  • ,
  • Ryo Nagao
  • ,
  • Yuki Kato
  • ,
  • Hiroshi Fukumura
  • ,
  • Yutaka Shibata
  • ,
  • Takumi Noguchi

198
開始ページ
121
終了ページ
134
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1039/c6fd00188b
出版者・発行元
ROYAL SOC CHEMISTRY

Development of an efficient photo-anode system for water oxidation is key to the success of artificial photosynthesis. We previously assembled photosystem II (PSII) proteins, which are an efficient natural photocatalyst for water oxidation, on a gold nanoparticle (GNP) to prepare a PSII-GNP conjugate as an anode system in a light-driven water-splitting nanodevice (Noji et al., J. Phys. Chem. Lett., 2011, 2, 2448-2452). In the current study, we characterized the fluorescence property of the PSII-GNP conjugate by static and time-resolved fluorescence measurements, and compared with that of free PSII proteins. It was shown that in a static fluorescence spectrum measured at 77 K, the amplitude of a major peak at 683 nm was significantly reduced and a red shoulder at 693 nm disappeared in PSII-GNP. Time-resolved fluorescence measurements showed that picosecond components at 683 nm decayed faster by factors of 1.4-2.1 in PSII-GNP than in free PSII, explaining the observed quenching of the major fluorescence peak. In addition, a nanosecond-decay component arising from a 'red chlorophyll' at 693 nm was lost in time-resolved fluorescence of PSII-GNP, probably due to a structural perturbation of this chlorophyll by interaction with GNP. Consistently with these fluorescence properties, degradation of PSII during strong-light illumination was two times slower in PSII-GNP than in free PSII. The enhanced durability of PSII is an advantageous property of the PSII-GNP conjugate in the development of an artificial photosynthesis device.

リンク情報
DOI
https://doi.org/10.1039/c6fd00188b
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000402870300007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1039/c6fd00188b
  • ISSN : 1359-6640
  • eISSN : 1364-5498
  • Web of Science ID : WOS:000402870300007

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