2021年1月
Molecular organizations and function of iron-stress-induced-A protein family in Anabaena sp. PCC 7120
Biochimica et Biophysica Acta (BBA) - Bioenergetics
- 巻
- 1862
- 号
- 1
- 開始ページ
- 148327
- 終了ページ
- 148327
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bbabio.2020.148327
- 出版者・発行元
- Elsevier BV
Iron-stress-induced-A proteins (IsiAs) are expressed in cyanobacteria under iron-deficient conditions, and surround photosystem I (PSI) trimer with a ring formation. A cyanobacterium Anabaena sp. PCC 7120 has four isiA genes; however, it is unknown how the IsiAs are associated with PSI. Here we report on molecular organizations and function of the IsiAs in this cyanobacterium. A deletion mutant of the isiA1 gene was constructed, and the four types of thylakoids were prepared from the wild-type (WT) and ΔisiA1 cells under iron-replete (+Fe) and iron-deficient (-Fe) conditions. Immunoblotting analysis exhibits a clear expression of the IsiA1 in the WT-Fe. The PSI-IsiA1 supercomplex is found in the WT-Fe, and excitation-energy transfer from IsiA1 to PSI is verified by time-resolved fluorescence analyses. Instead of the IsiA1, both IsiA2 and IsiA3 are bound to PSI monomer in the ΔisiA1-Fe. These findings provide insights into multiple-expression system of the IsiA family in this cyanobacterium.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.bbabio.2020.148327
- ISSN : 0005-2728
- PubMed ID : 33069682