論文

査読有り 筆頭著者 責任著者 国際誌
2021年1月

Molecular organizations and function of iron-stress-induced-A protein family in Anabaena sp. PCC 7120

Biochimica et Biophysica Acta (BBA) - Bioenergetics
  • Ryo Nagao
  • Makio Yokono
  • Yoshifumi Ueno
  • Takehiro Suzuki
  • Koji Kato
  • Ka-Ho Kato
  • Naoki Tsuboshita
  • Tian-Yi Jiang
  • Naoshi Dohmae
  • Jian-Ren Shen
  • Shigeki Ehira
  • Seiji Akimoto
  • 全て表示

1862
1
開始ページ
148327
終了ページ
148327
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbabio.2020.148327
出版者・発行元
Elsevier BV

Iron-stress-induced-A proteins (IsiAs) are expressed in cyanobacteria under iron-deficient conditions, and surround photosystem I (PSI) trimer with a ring formation. A cyanobacterium Anabaena sp. PCC 7120 has four isiA genes; however, it is unknown how the IsiAs are associated with PSI. Here we report on molecular organizations and function of the IsiAs in this cyanobacterium. A deletion mutant of the isiA1 gene was constructed, and the four types of thylakoids were prepared from the wild-type (WT) and ΔisiA1 cells under iron-replete (+Fe) and iron-deficient (-Fe) conditions. Immunoblotting analysis exhibits a clear expression of the IsiA1 in the WT-Fe. The PSI-IsiA1 supercomplex is found in the WT-Fe, and excitation-energy transfer from IsiA1 to PSI is verified by time-resolved fluorescence analyses. Instead of the IsiA1, both IsiA2 and IsiA3 are bound to PSI monomer in the ΔisiA1-Fe. These findings provide insights into multiple-expression system of the IsiA family in this cyanobacterium.

リンク情報
DOI
https://doi.org/10.1016/j.bbabio.2020.148327
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33069682
ID情報
  • DOI : 10.1016/j.bbabio.2020.148327
  • ISSN : 0005-2728
  • PubMed ID : 33069682

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