2014年8月
Single-Cell Imaging of Caspase-1 Dynamics Reveals an All-or-None Inflammasome Signaling Response
CELL REPORTS
- 巻
- 8
- 号
- 4
- 開始ページ
- 974
- 終了ページ
- 982
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.celrep.2014.07.012
- 出版者・発行元
- CELL PRESS
Inflammasome-mediated caspase-1 activation is involved in cell death and the secretion of the proinflammatory cytokine interleukin-1 beta (IL-1 beta). Although the dynamics of caspase-1 activation, IL-1 beta secretion, and cell death have been examined with bulk assays in population-level studies, they remain poorly understood at the single-cell level. In this study, we conducted single-cell imaging using a genetic fluorescence resonance energy transfer sensor that detects caspase-1 activation. We determined that caspase-1 exhibits all-or-none (digital) activation at the single-cell level, with similar activation kinetics irrespective of the type of inflammasome or the intensity of the stimulus. Real-time concurrent detection of caspase-1 activation and IL-1 beta release demonstrated that dead macrophages containing activated caspase-1 release a local burst of IL-1 beta in a digital manner, which identified these macrophages as the main source of IL-1 beta within cell populations. Our results highlight the value of single-cell analysis in enhancing understanding of the inflammasome system and chronic inflammatory diseases.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.celrep.2014.07.012
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000341573500007&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-84908356704&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0003-0493-4815
- ID情報
-
- DOI : 10.1016/j.celrep.2014.07.012
- ISSN : 2211-1247
- ORCIDのPut Code : 22666679
- SCOPUS ID : 84908356704
- Web of Science ID : WOS:000341573500007