論文

査読有り
2018年5月1日

Phosphatidylserine-stimulated production of N-acyl-phosphatidylethanolamines by Ca2+-dependent N-acyltransferase

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
  • Zahir Hussain
  • ,
  • Toru Uyama
  • ,
  • Katsuhisa Kawai
  • ,
  • Smriti Sultana Binte Mustafiz
  • ,
  • Kazuhito Tsuboi
  • ,
  • Nobukazu Araki
  • ,
  • Natsuo Ueda

1863
5
開始ページ
493
終了ページ
502
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbalip.2018.02.002
出版者・発行元
Elsevier B.V.

N-acyl-phosphatidylethanolamine (NAPE) is known to be a precursor for various bioactive N-acylethanolamines including the endocannabinoid anandamide. NAPE is produced in mammals through the transfer of an acyl chain from certain glycerophospholipids to phosphatidylethanolamine (PE) by Ca2+-dependent or -independent N-acyltransferases. The ε isoform of mouse cytosolic phospholipase A2 (cPLA2ε) was recently identified as a Ca2+-dependent N-acyltransferase (Ca-NAT). In the present study, we first showed that two isoforms of human cPLA2ε function as Ca-NAT. We next purified both mouse recombinant cPLA2ε and its two human orthologues to examine their catalytic properties. The enzyme absolutely required Ca2+ for its activity and the activity was enhanced by phosphatidylserine (PS). PS enhanced the activity 25-fold in the presence of 1 mM CaCl2 and lowered the EC50 value of Ca2+ &gt
8-fold. Using a PS probe, we showed that cPLA2ε largely co-localizes with PS in plasma membrane and organelles involved in the endocytic pathway, further supporting the interaction of cPLA2ε with PS in living cells. Finally, we found that the Ca2+-ionophore ionomycin increased [14C]NAPE levels &gt
10-fold in [14C]ethanolamine-labeled cPLA2ε-expressing cells while phospholipase A/acyltransferase-1, acting as a Ca2+-independent N-acyltransferase, was insensitive to ionomycin for full activity. In conclusion, PS potently stimulated the Ca2+-dependent activity and human cPLA2ε isoforms also functioned as Ca-NAT.

リンク情報
DOI
https://doi.org/10.1016/j.bbalip.2018.02.002
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29447909
ID情報
  • DOI : 10.1016/j.bbalip.2018.02.002
  • ISSN : 1879-2618
  • ISSN : 1388-1981
  • PubMed ID : 29447909
  • SCOPUS ID : 85042200722

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