Sep, 2014
A rapid and enhanced DNA detection method for crop cultivar discrimination
JOURNAL OF BIOTECHNOLOGY
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- Volume
- 185
- Number
- First page
- 57
- Last page
- 62
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.jbiotec.2014.06.013
- Publisher
- ELSEVIER SCIENCE BV
In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products. (C) 2014 The Authors. Published by Elsevier B.V.
- Link information
- ID information
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- DOI : 10.1016/j.jbiotec.2014.06.013
- ISSN : 0168-1656
- eISSN : 1873-4863
- Web of Science ID : WOS:000341046100009