論文

査読有り
2020年4月

Time-lapse imaging of HeLa spheroids in soft agar culture provides virtual inner proliferative activity

PLoS ONE
  • Minamikawa-Tachino R
  • ,
  • Ogura K
  • ,
  • Ito A
  • ,
  • Nagayama K

15
4
開始ページ
e0231774
終了ページ
e0231774
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0231774

© 2020 Minamikawa-Tachino et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Cancer is a complex disease caused by multiple types of interactions. To simplify and normalize the assessment of drug effects, spheroid microenvironments have been utilized. Research models that involve agent measurement with the examination of clonogenic survival by monitoring culture process with image analysis have been developed for spheroid-based screening. Meanwhile, computer simulations using various models have enabled better predictions for phenomena in cancer. However, user-based parameters that are specific to a researcher’s own experimental conditions must be inputted. In order to bridge the gap between experimental and simulated conditions, we have developed an in silico analysis method with virtual three-dimensional embodiment computed using the researcher’s own samples. The present work focused on HeLa spheroid growth in soft agar culture, with spheroids being modeled in silico based on time-lapse images capturing spheroid growth. The spheroids in silico were optimized by adjusting the growth curves to those obtained from time-lapse images of spheroids and were then assigned virtual inner proliferative activity by using generations assigned to each cellular particle. The ratio and distribution of the virtual inner proliferative activities were confirmed to be similar to the proliferation zone ratio and histochemical profiles of HeLa spheroids, which were also consistent with those identified in an earlier study. We validated that time-lapse images of HeLa spheroids provided virtual inner proliferative activity for spheroids in vitro. The present work has achieved the first step toward an in silico analysis method using computational simulation based on a researcher’s own samples, helping to bridge the gap between experiment and simulation.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0231774
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083489853&origin=inward
ID情報
  • DOI : 10.1371/journal.pone.0231774
  • eISSN : 1932-6203

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