論文

査読有り 国際誌
2019年3月29日

Increased flux in acetyl-CoA synthetic pathway and TCA cycle of Kluyveromyces marxianus under respiratory conditions.

Scientific reports
  • Yuri Sakihama
  • ,
  • Ryota Hidese
  • ,
  • Tomohisa Hasunuma
  • ,
  • Akihiko Kondo

9
1
開始ページ
5319
終了ページ
5319
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-019-41863-1

Yeasts are extremely useful, not only for fermentation but also for a wide spectrum of fuel and chemical productions. We analyzed the overall metabolic turnover and transcript dynamics in glycolysis and the TCA cycle, revealing the difference in adaptive pyruvate metabolic response between a Crabtree-negative species, Kluyveromyces marxianus, and a Crabtree-positive species, Saccharomyces cerevisiae, during aerobic growth. Pyruvate metabolism was inclined toward ethanol production under aerobic conditions in S. cerevisiae, while increased transcript abundances of the genes involved in ethanol metabolism and those encoding pyruvate dehydrogenase were seen in K. marxianus, indicating the augmentation of acetyl-CoA synthesis. Furthermore, different metabolic turnover in the TCA cycle was observed in the two species: malate and fumarate production in S. cerevisiae was higher than in K. marxianus, irrespective of aeration; however, fluxes of both the reductive and oxidative TCA cycles were enhanced in K. marxianus by aeration, implying both the cycles contribute to efficient electron flux without producing ethanol. Additionally, decreased hexokinase activity under aerobic conditions is expected to be important for maintenance of suitable carbon flux. These findings demonstrate differences in the key metabolic trait of yeasts employing respiration or fermentation, and provide important insight into the metabolic engineering of yeasts.

リンク情報
DOI
https://doi.org/10.1038/s41598-019-41863-1
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30926897
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440987

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