論文

査読有り
2020年7月8日

De novo transcriptome analysis reveals an unperturbed transcriptome under high cadmium conditions in the Cd-hypertolerant fern Athyrium yokoscense.

Genes & genetic systems
  • Yuko Ukai
  • Komaki Inoue
  • Manaka Kamada
  • Hiroshi Teramura
  • Shunsuke Yanagisawa
  • Kazuyoshi Kitazaki
  • Kazuhiro Shoji
  • Fumiyuki Goto
  • Keiichi Mochida
  • Toshihiro Yoshihara
  • Hiroaki Shimada
  • 全て表示

95
2
開始ページ
65
終了ページ
74
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1266/ggs.19-00056

Athyrium yokoscense shows strong tolerance to cadmium exposure, even at levels that are many times greater than the toxic levels in ordinary plants. To determine the mechanism of Cd tolerance in A. yokoscense, we grew these plants under high Cd conditions and observed the tissue-specific accumulation of Cd and generation of reactive oxygen species, which is one of the major physiological responses to Cd stress. Fuchsin staining indicated the existence of a casparian strip in A. yokoscense roots, which may participate in Cd hypertolerance in A. yokoscense. Moreover, we performed RNA-seq of RNA samples from A. yokoscense plants treated with or without Cd exposure and obtained comprehensive RNA sequences as well as the Cd-responsive expression patterns of individual genes. Through de novo transcriptome assembly and gene expression analysis, we found that A. yokoscense showed normal features with no significant change in the expression levels of any transporter genes, even under high Cd exposure conditions. Our results demonstrate that A. yokoscense has an unusual mechanism that allows the invading Cd to partition into the distal roots, thus avoiding translocation of Cd into the xylem.

リンク情報
DOI
https://doi.org/10.1266/ggs.19-00056
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32389921
ID情報
  • DOI : 10.1266/ggs.19-00056
  • PubMed ID : 32389921

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