論文

2010年3月

Community shifts of soybean stem-associated bacteria responding to different nodulation phenotypes and N levels

ISME JOURNAL
  • Seishi Ikeda
  • ,
  • Takashi Okubo
  • ,
  • Takakazu Kaneko
  • ,
  • Shoko Inaba
  • ,
  • Tomiya Maekawa
  • ,
  • Shima Eda
  • ,
  • Shusei Sato
  • ,
  • Satoshi Tabata
  • ,
  • Hisayuki Mitsui
  • ,
  • Kiwamu Minamisawa

4
3
開始ページ
315
終了ページ
326
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/ismej.2009.119
出版者・発行元
NATURE PUBLISHING GROUP

The diversity of stem-associated bacteria of non-nodulated (Nod(-)), wild-type nodulated (Nod(+)) and hypernodulated (Nod(++)) soybeans were evaluated by clone library analyses of the 16S ribosomal RNA gene. Soybeans were dressed with standard nitrogen (SN) fertilization (15 kg N ha(-1)) and heavy nitrogen (HN) fertilization (615 kg N ha(-1)). The relative abundance of Alphaproteobacteria in Nod(+) soybeans (66%) was smaller than that in Nod(-) and Nod(++) soybeans (75-76%) under SN fertilization, whereas that of Gammaproteobacteria showed the opposite pattern (23% in Nod(+) and 12-16% in Nod(-) and Nod(++) soybeans). Principal coordinate analysis showed that the bacterial communities of Nod(-) and Nod(++) soybeans were more similar to each other than to that of Nod(+) soybeans under SN fertilization. HN fertilization increased the relative abundance of Gammaproteobacteria in all nodulation phenotypes (33-57%) and caused drastic shifts of the bacterial community. The clustering analyses identified a subset of operational taxonomic units (OTUs) at the species level in Alpha-and Gammaproteobacteria responding to both the nodulation phenotypes and nitrogen fertilization levels. Meanwhile, the abundance of Betaproteobacteria was relatively constant in all libraries constructed under these environmental conditions. The relative abundances of two OTUs in Alphaproteobacteria (Aurantimonas sp. and Methylobacterium sp.) were especially sensitive to nodulation phenotype and were drastically decreased under HN fertilization. These results suggested that a subpopulation of proteobacteria in soybeans is controlled in a similar manner through both the regulation systems of plant-rhizobia symbiosis and the nitrogen signaling pathway in plants. The ISME Journal (2010) 4, 315-326; doi:10.1038/ismej.2009.119; published online 19 November 2009

リンク情報
DOI
https://doi.org/10.1038/ismej.2009.119
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000274800100001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/ismej.2009.119
  • ISSN : 1751-7362
  • Web of Science ID : WOS:000274800100001

エクスポート
BibTeX RIS