2016年4月
Haspin has Multiple Functions in the Plant Cell Division Regulatory Network
PLANT AND CELL PHYSIOLOGY
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- 巻
- 57
- 号
- 4
- 開始ページ
- 848
- 終了ページ
- 861
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/pcp/pcw030
- 出版者・発行元
- OXFORD UNIV PRESS
Progression of cell division is controlled by various mitotic kinases. In animal cells, phosphorylation of histone H3 at Thr3 by the kinase Haspin (haploid germ cell-specific nuclear protein kinase) promotes centromeric Aurora B localization to regulate chromosome segregation. However, less is known about the function of Haspin in regulatory networks in plant cells. Here, we show that inhibition of Haspin with 5-iodotubercidin (5-ITu) in Bright Yellow-2 (BY-2) cells delayed chromosome alignment. Haspin inhibition also prevented the centromeric localization of Aurora3 kinase (AUR3) and disrupted its function. This suggested that Haspin plays a role in the specific positioning of AUR3 on chromosomes in plant cells, a function conserved in animals. The results also indicated that Haspin and AUR3 are involved in the same pathway, which regulates chromosome alignment during prometaphase/metaphase. Remarkably, Haspin inhibition by 5-ITu also led to a severe cytokinesis defect, resulting in binuclear cells with a partially formed cell plate. The 5-ITu treatment did not affect microtubules, AUR1/2 or the NACK-PQR pathway; however, it did alter the distribution of actin filaments on the cell plate. Together, these results suggested that Haspin has several functions in regulating cell division in plant cells: in the localization of AUR3 on centromeres and in regulating late cell plate expansion during cytokinesis.
- リンク情報
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- DOI
- https://doi.org/10.1093/pcp/pcw030
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/26872832
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000376654100017&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/26872832
- URL
- http://orcid.org/0000-0003-2703-0405
- ID情報
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- DOI : 10.1093/pcp/pcw030
- ISSN : 0032-0781
- eISSN : 1471-9053
- ORCIDのPut Code : 23675062
- PubMed ID : 26872832
- Web of Science ID : WOS:000376654100017