論文

査読有り
2016年11月

Combination of Synthetic Chemistry and Live-Cell Imaging Identified a Rapid Cell Division Inhibitor in Tobacco and Arabidopsis thaliana.

Plant & cell physiology
  • Masakazu Nambo
  • ,
  • Daisuke Kurihara
  • ,
  • Tomomi Yamada
  • ,
  • Taeko Nishiwaki-Ohkawa
  • ,
  • Naoya Kadofusa
  • ,
  • Yusuke Kimata
  • ,
  • Keiko Kuwata
  • ,
  • Masaaki Umeda
  • ,
  • Minako Ueda

57
11
開始ページ
2255
終了ページ
2268
記述言語
英語
掲載種別
DOI
10.1093/pcp/pcw140

Cell proliferation is crucial to the growth of multicellular organisms, and thus the proper control of cell division is important to prevent developmental arrest or overgrowth. Nevertheless, tools for controlling cell proliferation are still poor in plant. To develop novel tools, we focused on a specific compound family, triarylmethanes, whose members show various antiproliferative activities in animals. By combining organic chemistry to create novel and diverse compounds containing the triarylmethyl moiety and biological screens based on live-cell imaging of a fluorescently labeled tobacco Bright Yellow-2 (BY-2) culture cell line (Nicotiana tabacum), we isolated (3-furyl)diphenylmethane as a strong but partially reversible inhibitor of plant cell division. We also found that this agent had efficient antiproliferative activity in developing organs of Arabidopsis thaliana without causing secondary defects in cell morphology, and induced rapid cell division arrest independent of the cell cycle stage. Given that (3-furyl)diphenylmethane did not affect the growth of a human cell line (HeLa) and a budding yeast (Saccharomyces cerevisiae), it should act specifically on plants. Taking our results together, we propose that the combination of desired chemical synthesis and detailed biological analysis is an effective tool to create novel drugs, and that (3-furyl)diphenylmethane is a specific antiproliferative agent for plants.

リンク情報
DOI
https://doi.org/10.1093/pcp/pcw140
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27519314
ID情報
  • DOI : 10.1093/pcp/pcw140
  • eISSN : 1471-9053
  • PubMed ID : 27519314

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