2017年6月
Fluorescent Labeling of the Cyst Nematode Heterodera glycines in Deep-Tissue Live Imaging
CYTOLOGIA
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- 巻
- 82
- 号
- 3
- 開始ページ
- 251
- 終了ページ
- 259
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1508/cytologia.82.251
- 出版者・発行元
- UNIV TOKYO CYTOLOGIA
Nematode infection of plant roots is a paradigm of host parasite interactions. Although nematodes can be labeled with fluorescent dyes, migration of the worms into the deep regions of host roots makes them difficult to track. Here we report the use of two fluorescent dyes, FM4-64 and SYBR green I, to intensely label the soybean cyst nematode (SCN) Heterodera glycines for one week in host plants. Continuous monitoring of the labeled SCN juveniles was achieved with two-photon microscopy. Additionally, we developed a transient transformation system consisting of the non-model leguminous plant (fabaceous) roots, Astragalus sinicus and Agrobacterium rhizogenes to observe the cellular structures of the plant during SCN infection. By the combined use of fluorescent dyes and two-photon microscopy, clear images of infecting SCNs were obtained even in deep regions of A. sinicus roots. The fluorescent labeling described herein can also be used in detailed monitoring of the infection processes of other non-model nematodes, as well as the associated morphological changes in the host plant roots.
- リンク情報
- ID情報
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- DOI : 10.1508/cytologia.82.251
- ISSN : 0011-4545
- Web of Science ID : WOS:000410719400007