論文

査読有り 招待有り 最終著者 責任著者
2021年8月19日

In vivo wide-field voltage imaging in zebrafish with voltage-sensitive dye and genetically encoded voltage indicator.

Development, growth & differentiation
  • Kanae Hiyoshi
  • ,
  • Asuka Shiraishi
  • ,
  • Narumi Fukuda
  • ,
  • Sachiko Tsuda

記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/dgd.12744

The brain consists of neural circuits, which are assemblies of various neuron types. For understanding how the brain works, it is essential to identify the functions of each type of neuron and neuronal circuits. Recent advances in our understanding of brain function and its development have been achieved using light to detect neuronal activity. Optical measurement of membrane potentials through voltage imaging is a desirable approach, enabling fast, direct, and simultaneous detection of membrane potentials in a population of neurons. Its high speed and directness can help detect synaptic and action potentials and hyperpolarization, which encode critical information for brain function. Here, we describe in vivo voltage imaging procedures that we have recently established using zebrafish, a powerful animal model in developmental biology and neuroscience. By applying two types of voltage sensors, voltage-sensitive dyes (VSDs, Di-4-ANEPPS) and genetically encoded voltage indicators (GEVIs, ASAP1), spatiotemporal dynamics of voltage signals can be detected in the whole cerebellum and spinal cord in awake fish at single-cell and neuronal population levels. Combining this method with other approaches, such as optogenetics, behavioral analysis, and electrophysiology would facilitate a deeper understanding of the network dynamics of the brain circuitry and its development.

リンク情報
DOI
https://doi.org/10.1111/dgd.12744
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34411280
ID情報
  • DOI : 10.1111/dgd.12744
  • PubMed ID : 34411280

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