Knut Woltjen

J-GLOBAL         Last updated: Jun 14, 2019 at 11:51
 
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Name
Knut Woltjen
Affiliation
Kyoto University
Section
Center for iPS Cell Research and Application(CiRA)
Job title
Associate Professor
ORCID ID
0000-0003-2293-1183

Research Areas

 

Academic & Professional Experience

 
Apr 2013
 - 
Mar 2018
Associate Professor, Center for iPS Cell Research and Application (CiRA)/ Hakubi Center for Advanced Research, Kyoto University
 
Apr 2010
 - 
Mar 2013
Assistant Professor, Center for iPS Cell Research and Application (CiRA), Kyoto University
 
Feb 2009
 - 
Mar 2010
iPS Cell Facility Manager, The Hospital For Sick Children, Ontario Human iPS Cell Facility
 
Sep 2006
 - 
Mar 2010
Postdoctoral Research Fellow, Dr. Andras Nagy Lab, Mount Sinai Hospital, Samuel Lunenfeld Research Institute
 
Jan 2005
 - 
Aug 2006
Molecular/Gene Targeting Consultant, Centre for Mouse Genomics, University of Calgary
 

Education

 
Sep 2000
 - 
Apr 2006
Dept. Biochemistry and Molecular Biology, Doctoral Research Program, University of Calgary
 
Sep 1998
 - 
Sep 2000
Dept. Biochemistry and Molecular Biology, Masters Research Program, University of Calgary
 
Sep 1994
 - 
Apr 1998
Dept. of Biological Sciences, Faculty of Genetics, Molecular Genetics Program, University of Alberta
 

Committee Memberships

 
Jul 2018
 - 
Today
Japanese Society for Genome Editing  International Committee
 
Jul 2016
 - 
Today
Japanese Society for Genome Editing  Public Relations Committee
 

Published Papers

 
Yagi M, Kabata M, Ukai T, Ohta S, Tanaka A, Shimada Y, Sugimoto M, Araki K, Okita K, Woltjen K, Hochedlinger K, Yamamoto T, Yamada Y
Stem cell reports   12(5) 1113-1128   May 2019   [Refereed]
Jo N, Sogabe Y, Yamada Y, Ukai T, Kagawa H, Mitsunaga K, Woltjen K, Yamada Y
Cancer science   110(3) 926-938   Mar 2019   [Refereed]
Kagawa H, Shimamoto R, Kim SI, Oceguera-Yanez F, Yamamoto T, Schroeder T, Woltjen K
Stem cell reports   12(2) 319-332   Feb 2019   [Refereed]
Woltjen K, Bortvin A
Current opinion in genetics & development   52 ii-iv   Oct 2018   [Refereed]
Shibata H, Komura S, Yamada Y, Sankoda N, Tanaka A, Ukai T, Kabata M, Sakurai S, Kuze B, Woltjen K, Haga H, Ito Y, Kawaguchi Y, Yamamoto T, Yamada Y
Nature communications   9(1) 2081   May 2018   [Refereed]
Ikeda T, Hikichi T, Miura H, Shibata H, Mitsunaga K, Yamada Y, Woltjen K, Miyamoto K, Hiratani I, Yamada Y, Hotta A, Yamamoto T, Okita K, Masui S
Nature communications   9(1) 1387   Apr 2018   [Refereed]
Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K
Nature communications   9(1) 939   Mar 2018   [Refereed]
Kondo T, Imamura K, Funayama M, Tsukita K, Miyake M, Ohta A, Woltjen K, Nakagawa M, Asada T, Arai T, Kawakatsu S, Izumi Y, Kaji R, Iwata N, Inoue H
Cell reports   21(8) 2304-2312   Nov 2017   [Refereed]
Kojima Y, Sasaki K, Yokobayashi S, Sakai Y, Nakamura T, Yabuta Y, Nakaki F, Nagaoka S, Woltjen K, Hotta A, Yamamoto T, Saitou M
Cell stem cell   21(4) 517-532.e5   Oct 2017   [Refereed]
Kieffer TJ, Woltjen K, Osafune K, Yabe D, Inagaki N
Journal of diabetes investigation      Oct 2017   [Refereed]
Hirosawa M, Fujita Y, Parr CJC, Hayashi K, Kashida S, Hotta A, Woltjen K, Saito H
Nucleic acids research   45(13) e118   Jul 2017   [Refereed]
Imamura K, Izumi Y, Watanabe A, Tsukita K, Woltjen K, Yamamoto T, Hotta A, Kondo T, Kitaoka S, Ohta A, Tanaka A, Watanabe D, Morita M, Takuma H, Tamaoka A, Kunath T, Wray S, Furuya H, Era T, Makioka K, Okamoto K, Fujisawa T, Nishitoh H, Homma K, Ichijo H, Julien JP, Obata N, Hosokawa M, Akiyama H, Kaneko S, Ayaki T, Ito H, Kaji R, Takahashi R, Yamanaka S, Inoue H
Science translational medicine   9(391)    May 2017   [Refereed]
Sone M, Morone N, Nakamura T, Tanaka A, Okita K, Woltjen K, Nakagawa M, Heuser JE, Yamada Y, Yamanaka S, Yamamoto T
Cell metabolism   25(5) 1103-1117.e6   May 2017   [Refereed]
Hashimoto K, Yamada Y, Semi K, Yagi M, Tanaka A, Itakura F, Aoki H, Kunisada T, Woltjen K, Haga H, Sakai Y, Yamamoto T, Yamada Y
Proceedings of the National Academy of Sciences of the United States of America   114(4) 758-763   Jan 2017   [Refereed]
Woltjen K, Yamamoto T, Kokubu C, Takeda J
Genes to cells : devoted to molecular & cellular mechanisms   21(5) 392-395   May 2016   [Refereed]
Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
Methods (San Diego, Calif.)   101 43-55   May 2016   [Refereed]
Komura S, Semi K, Itakura F, Shibata H, Ohno T, Hotta A, Woltjen K, Yamamoto T, Akiyama H, Yamada Y
Stem cell reports   6(4) 592-606   Apr 2016   [Refereed]
Woltjen K, Kim SI, Nagy A
Methods in molecular biology (Clifton, N.J.)   1357 1-22   2016   [Refereed]
Chantzoura E, Skylaki S, Menendez S, Kim SI, Johnsson A, Linnarsson S, Woltjen K, Chambers I, Kaji K
Stem cell reports   5(3) 350-364   Sep 2015   [Refereed]
Sasaki K, Yokobayashi S, Nakamura T, Okamoto I, Yabuta Y, Kurimoto K, Ohta H, Moritoki Y, Iwatani C, Tsuchiya H, Nakamura S, Sekiguchi K, Sakuma T, Yamamoto T, Mori T, Woltjen K, Nakagawa M, Yamamoto T, Takahashi K, Yamanaka S, Saitou M
Cell stem cell   17(2) 178-194   Aug 2015   [Refereed]
Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, Yamamoto T, Yamanaka S, Woltjen K.
Stem Cell Reports   4(4) 727-743   Apr 2015   [Refereed]
Matsuda M, Koga M, Woltjen K, Nishida E, Ebisuya M.
Nature Communications   6(6195)    2015   [Refereed]
Tamaki S, Fukuta M, Sekiguchi K, Jin Y, Nagata S, Hayakawa K, Hineno S, Okamoto T, Watanabe M, Woltjen K, Ikeya M, Kato T Jr, Toguchida J
PloS one   10(11) e0142991   2015   [Refereed]
Jin Y, Elalaf H, Watanabe M, Tamaki S, Hineno S, Matsunaga K, Woltjen K, Kobayashi Y, Nagata S, Ikeya M, Kato T Jr, Okamoto T, Matsuda S, Toguchida J
PloS one   10(7) e0131998   2015   [Refereed]
Shoji E, Woltjen K, Sakurai H.
Methods in Molecular Biology   1353 89-99   2015   [Refereed][Invited]
Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen K
Methods in Molecular Biology   1357 111-131   2015   [Refereed][Invited]
K. Ohnishi; K. Semi; T. Yamamoto; M. Shimizu; A. Tanaka; K. Mitsunaga; K. Okita; K. Osafune; Y. Arioka; T. Maeda; H. Soejima; H. Moriwaki; S. Yamanaka; K. Woltjen; Y. Yamada
Cell   156(4) 663-677   Feb 2014   [Refereed]
T. Sakuma; K. Woltjen
Development Growth and Differentiation   56(1) 2-13   Jan 2014   [Refereed]
Gallagher D, Norman AA, Woodard CL, Yang G, Gauthier-Fisher A, Fujitani M, Vessey JP, Cancino GI, Sachewsky N, Woltjen K, Fatt MP, Morshead CM, Kaplan DR, Miller FD.
Cell Stem Cell   13(5) 564-576   Nov 2013   [Refereed]
T. Sakuma; S. Hosoi; K. Woltjen; K.-I. Suzuki; K. Kashiwagi; H. Wada; H. Ochiai; T. Miyamoto; N. Kawai; Y. Sasakura; S. Matsuura; Y. Okada; A. Kawahara; S. Hayashi; T. Yamamoto
Genes to Cells   18(4) 315-326   Apr 2013   [Refereed]
K. Hayakawa; M. Ikeya; M. Fukuta; K. Woltjen; S. Tamaki; N. Takahara; T. Kato; S. Sato; T. Otsuka; J. Toguchida
Biochemical and Biophysical Research Communications   432(4) 713-719   Mar 2013   [Refereed]
A. Tanaka; K. Woltjen; K. Miyake; A. Hotta; M. Ikeya; T. Yamamoto; T. Nishino; E. Shoji; A. Sehara-Fujisawa; Y. Manabe; N. Fujii; K. Hanaoka; T. Era; S. Yamashita; K.-I. Isobe; E. Kimura; H. Sakurai
PLoS ONE   8(4) e61540   2013   [Refereed]
A. Yamashita; S. Liu; K. Woltjen; B. Thomas; G. Meng; A. Hotta; K. Takahashi; J. Ellis; S. Yamanaka; D.E. Rancourt
Scientific Reports   3 1978   2013   [Refereed]
I.P. Michael; C. Monetti; A.C. Chiu; P. Zhang; T. Baba; K. Nishino; S. Agha-Mohammadi; K. Woltjen; H.-K. Sung; A. Nagy
Molecular Cancer   11 89   Dec 2012   [Refereed]
K. Nagy; H.-K. Sung; P. Zhang; S. Laflamme; P. Vincent; S. Agha-Mohammadi; K. Woltjen; C. Monetti; I.P. Michael; L.C. Smith; A. Nagy
Stem Cell Reviews and Reports   8(2) 546-   2012   [Refereed]
K. Nagy; H.-K. Sung; P. Zhang; S. Laflamme; P. Vincent; S. Agha-Mohammadi; K. Woltjen; C. Monetti; I.P. Michael; L.C. Smith; A. Nagy
Stem Cell Reviews and Reports   7(3) 693-702   Sep 2011   [Refereed]
M. Gabut; P. Samavarchi-Tehrani; X. Wang; V. Slobodeniuc; D. O'Hanlon; H.-K. Sung; M. Alvarez; S. Talukder; Q. Pan; E.O. Mazzoni; S. Nedelec; H. Wichterle; K. Woltjen; T.R. Hughes; P.W. Zandstra; A. Nagy; J.L. Wrana; B.J. Blencowe
Cell   147(1) 132-146   Sep 2011   [Refereed]
C. Monetti; K. Nishino; S. Biechele; P. Zhang; T. Baba; K. Woltjen; A. Nagy
Methods   53(4) 380-385   Apr 2011   [Refereed]
Transposon-based production of genetically unmodified induced pluripotent stem cells from human embryonic fibroblasts
Woltjen K, Hamalainen R, Kibschull M, Mileikovsky M, Nagy A.
Methods in Molecular Biology 767 (87):103      2011   [Refereed]
K. Woltjen; R. Hämäläinen; M. Kibschull; M. Mileikovsky; A. Nagy
Methods in Molecular Biology   767 87-103   2011   [Refereed]
P. Samavarchi-Tehrani; A. Golipour; L. David; H.-K. Sung; T.A. Beyer; A. Datti; K. Woltjen; A. Nagy; J.L. Wrana
Cell Stem Cell   7(1) 64-77   Jul 2010   [Refereed]
Obtaining Pluripotency Biomarker Signatures
K. Sciabica; K. Woltjen; A. Hotta
Genetic Engineering and Biotechnology News   30(3)    2010
K. Woltjen; W.L. Stanford
Cell Stem Cell   5(5) 457-458   Nov 2009   [Refereed]
J. O'Malley; K. Woltjen; K. Kaji
Current Opinion in Biotechnology   20(5) 516-521   Oct 2009   [Refereed]
K. Woltjen; I.P. Michael; P. Mohseni; R. Desai; M. Mileikovsky; R. Hämäläinen; R. Cowling; W. Wang; P. Liu; M. Gertsenstein; K. Kaji; H.-K. Sung; A. Nagy
Nature   458(7239) 766-770   Apr 2009   [Refereed]
K. Kaji; K. Norrby; A. Paca; M. Mileikovsky; P. Mohseni; K. Woltjen
Nature   458(7239) 771-775   Apr 2009   [Refereed]
Woltjen K, Ito K, Tsuzuki T, Rancourt DE
Methods in molecular biology (Clifton, N.J.)   435 79-94   2008   [Refereed]
B. Thomas; K. Woltjen; D.E. Rancourt
BioTechniques   34(1) 36-8, 40   Jan 2003   [Refereed]
Bacteriophage gene targeting vectors generated by transplacement
C. Aoyama; K. Woltjen; F.C. Mansergh; K. Ishidate; D.E. Rancourt
BioTechniques   33(4) 806-812   2002   [Refereed]
Transplacement mutagenesis. A recombination-based in situ mutagenesis protocol.
K. Woltjen; M.W. Unger; D.E. Rancourt
Methods in molecular biology (Clifton, N.J.)   182 189-207   2002   [Refereed][Invited]

Misc

 
Establishment and functional \nanalysis of iPS cells with drug-inducible SYT-SSX fusion gene.
Fukuta M, Hayakawa K, Tamaki S, Sato S, Ikeya M, Otsuka Y, Woltjen K, Toguchida J.
第10 回ISSCR(2012.6.14.横浜)      2012   [Refereed]
Application of iPS cell technology \nfor the analysis of origin-unknown sarcoma characterized by a specific fusion gene.
Hayakawa K, Fukuta M, TamaiS, Kato T, Ikeya M, Otsuka T, Woltjen K, Toguchida J.
第17 回CTOS (2012.11.16.Prague)      Nov 2012   [Refereed]

Books etc

 
iPS細胞を用いた正確な ゲノム編集法(MhAX法)
Harunobu Kagawa, Tomoko Matsumoto, Shin-Il Kim, and Knut Woltjen (Part:Contributor, pp. 1-8)
実験医学   Oct 2018   

Conference Activities & Talks

 
Precise Human Disease Allele Creation and Correction through Microhomology-Mediated End Joining [Invited]
Knut Woltjen
2019 Keystone Symposia 『Genome Engineering: From Mechanisms to Therapies (B4)』   21 Feb 2019   
The history and potential of iPSC research [Invited]
Knut Woltjen
AAAS 2019 Annual Meeting   15 Feb 2019   
Japan’s experience in regenerative medicine and superdonor cells [Invited]
Knut Woltjen
NRC’s HEALTH CHALLENGE PROGRAM WORKSHOP : ‘Transforming Health Outcomes through Engineered Cell and Gene Therapies’   7 Dec 2018   
Precise Human Disease Allele Creation and Correction through Microhomology-Mediated End Joining [Invited]
Knut Woltjen
Concordia University Seminar   6 Dec 2018   
Precise human disease allele creation and correction through microhomology-mediated end joining [Invited]
Knut Woltjen
The 41st Annual Meeting of the Molecular Biology Society of Japan   28 Nov 2018   

Patents

 
特願PCT/IB2017/054736 : Method for genome editing
Knut Woltjen, Shin-Il Kim, Tomoko Matsumoto