An electron gas in a one-dimensional periodic potential can be transported even in the absence of a voltage bias if the potential is slowly and periodically modulated in time. Remarkably, the transferred charge per cycle is sensitive only to the topology of the path in parameter space. Although this so-called Thouless charge pump was first proposed more than thirty years ago(1), it has not yet been realized. Here we report the demonstration of topological Thouless pumping using ultracold fermionic atoms in a dynamically controlled optical superlattice. We observe a shift of the atomic cloud as a result of pumping, and extract the topological invariance of the pumping process from this shift. We demonstrate the topological nature of the Thouless pump by varying the topology of the pumping path and verify that the topological pump indeed works in the quantum regime by varying the speed and temperature.