論文

査読有り
1994年1月

FURIN HAS THE PROALBUMIN SUBSTRATE-SPECIFICITY AND SERPIN INHIBITORY PROPERTIES OF AN IN-SITU HEPATIC CONVERTASE

FEBS LETTERS
  • SO BRENNAN
  • ,
  • K NAKAYAMA

338
2
開始ページ
147
終了ページ
151
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/0014-5793(94)80353-6
出版者・発行元
ELSEVIER SCIENCE BV

Furin, a KEX2 protease homolog with high RNA expression in the liver is an excellent candidate as a hepatic proprotein convertase. Here we show that purified recombinant furin has the same proalbumin specificity and serpin inhibitory properties as the in situ hepatic convertase. There was rapid cleavage at the -RRD- site of normal human proalbumin but there no significant cleavage of natural unprocessed Variants with cleavage site sequences of -RRV-, -HRD-, -RQD-, or -CRD-. Cleavage of the latter was not increased by S-aminoethylation. Furin was specifically inhibited by alpha(1)-antitrypsin Pittsburgh (358 Met-->Arg), (K1/2 = 3 mu M) but not by 50 mu M normal antitrypsin M or by antithrombin, however, antithrombin/heparin was a good inhibitor (K1/2 = 9 mu M). The pH optimum for proalbumin cleavage was between pH 5.5 and 6.0, indicating that furin is potentially fully active within secretory vesicles, the site of proalbumin cleavage.

リンク情報
DOI
https://doi.org/10.1016/0014-5793(94)80353-6
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1994MV96600008&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-0028157378&partnerID=MN8TOARS
URL
http://orcid.org/0000-0001-7701-7183
ID情報
  • DOI : 10.1016/0014-5793(94)80353-6
  • ISSN : 0014-5793
  • ORCIDのPut Code : 44277970
  • SCOPUS ID : 0028157378
  • Web of Science ID : WOS:A1994MV96600008

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