2005年
Expression of BIG2 and analysis of its function in mammalian cells
GTPASES REGULATING MEMBRANE DYNAMICS
- ,
- ,
- 巻
- 404
- 号
- 開始ページ
- 206
- 終了ページ
- 215
- 記述言語
- 英語
- 掲載種別
- 論文集(書籍)内論文
- DOI
- 10.1016/S0076-6879(05)04020-6
- 出版者・発行元
- ELSEVIER ACADEMIC PRESS INC
BIG2 is a member of brefeldin A-inhibited guanine nucleotide exchange factors for ADP-ribosylation factors (ARFs). Although BIG2 is associated mainly with the trans-Golgi network, we have recently revealed that some population of BIG2 is associated with the recycling endosome. Moreover, we have found that expression of a catalytically inactive BIG2 mutant, E738K, selectively induces membrane tubules from this compartment. We have also demonstrated that the exchange activity of BIG2 is specific for class I ARFs (ARF1 and ARF3) in vivo and inactivation of either ARF enhances the BIG2(E738K)-induced membrane tubulation. Therefore, we have proposed that BIG2 is implicated in the structural integrity of the recycling endosome through activating class I ARFs. This article describes methods used for examining the function of BIG2 including basic protocols, which are not conventional because of cytotoxicity of BIG2 in Escherichia coli. cells and its low expression efficiency in mammalian cells.
- リンク情報
-
- DOI
- https://doi.org/10.1016/S0076-6879(05)04020-6
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16413271
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000234565700020&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-30544451376&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0001-7701-7183
- ID情報
-
- DOI : 10.1016/S0076-6879(05)04020-6
- ISSN : 0076-6879
- ORCIDのPut Code : 44277977
- PubMed ID : 16413271
- SCOPUS ID : 30544451376
- Web of Science ID : WOS:000234565700020