We have cloned a cDNA encoding rabbit alpha(1d) adrenoceptor from the rabbit liver cDNA library. The deduced amino-acid sequence of this clone encodes a protein of 576 amino acids that shows strong sequence homology to previously cloned human, rat and mouse alpha(1d) adrenoceptors. The pharmacological radioligand binding properties of this clone expressed in COS-7 cells were similar to those of rat alpha(1d)-adrenoceptors. Competitive RT/PCR assays revealed wide tissue distribution of the alpha(1d) adrenoceptor mRNA in rabbit, especially abundant in vas deferens, aorta, prostate and cerebral cortex.