Takashi Shinohara - My portal

Profile Information

Affiliation: Kyoto University

J-GLOBAL ID: 201401084271619658
researchmap Member ID: 7000008413

Research Areas

Life sciences / Laboratory animal science /

Papers

Allogeneic offspring produced by induction of PD-L1 in spermatogonial stem cells via self-renewal stimulation.

Takashi Shinohara, Takuya Yamamoto, Hiroko Morimoto, Yusuke Shiromoto, Mito Kanatsu-Shinohara

Stem cell reports, 18(4) 985-998, Apr 11, 2023
Glutamine protects mouse spermatogonial stem cells against NOX1-derived ROS for sustaining self-renewal division in vitro.

Takehiro Miyazaki, Mito Kanatsu-Shinohara, Narumi Ogonuki, Shogo Matoba, Atsuo Ogura, Chihiro Yabe-Nishimura, Hongliang Zhang, Yves Pommier, Andreas Trumpp, Takashi Shinohara

Development (Cambridge, England), Mar 8, 2023
Signal regulatory protein alpha is a conserved marker for mouse and rat spermatogonial stem cells.

Takehiro Miyazaki, Mito Kanatsu-Shinohara, Masatsugu Ema, Takashi Shinohara

Biology of reproduction, Jan 16, 2023
Adenovirus-mediated gene delivery restores fertility in congenitally infertile female mice.

Mito Kanatsu-Shinohara, Jiyoung Lee, Takehiro Miyazaki, Hiroko Morimoto, Takashi Shinohara

The Journal of reproduction and development, 68(6) 369-376, Dec 19, 2022
Regulation of male germline transmission patterns by the Trp53-Cdkn1a pathway.

Mito Kanatsu-Shinohara, Honda Naoki, Takashi Tanaka, Misako Tatehana, Takako Kikkawa, Noriko Osumi, Takashi Shinohara

Stem cell reports, 17(9) 1924-1941, Aug 1, 2022
Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice.

Mito Kanatsu-Shinohara, Jiyoung Lee, Takehiro Miyazaki, Hiroko Morimoto, Takashi Shinohara

Cell reports. Medicine, 3(5) 100606-100606, May 17, 2022
Regeneration of spermatogenesis by mouse germ cell transplantation into allogeneic and xenogeneic testis primordia or organoids.

Mito Kanatsu-Shinohara, Narumi Ogonuki, Shogo Matoba, Hiroko Morimoto, Yusuke Shiromoto, Atsuo Ogura, Takashi Shinohara

Stem cell reports, 17(4) 924-935, Apr 12, 2022
Genomic stability of mouse spermatogonial stem cells in vitro

Shinichiro Chuma, Mito Kanatsu-Shinohara, Ami Katanaya, Mihoko Hosokawa, Takashi Shinohara

Scientific Reports, 11(1), Dec, 2021
Cdc42 is required for male germline niche development in mice.

Yoshifumi Mori, Seiji Takashima, Mito Kanatsu-Shinohara, Zheng Yi, Takashi Shinohara

Cell reports, 36(7) 109550-109550, Aug 17, 2021
Spermatogonial stem cell transplantation into nonablated mouse recipient testes.

Hiroko Morimoto, Narumi Ogonuki, Mito Kanatsu-Shinohara, Shogo Matoba, Atsuo Ogura, Takashi Shinohara

Stem cell reports, 16(7) 1832-1844, Jul 13, 2021
OGG1 protects mouse spermatogonial stem cells from reactive oxygen species in culture†.

Yoshifumi Mori, Narumi Ogonuki, Ayumi Hasegawa, Mito Kanatsu-Shinohara, Atsuo Ogura, Yufeng Wang, John R McCarrey, Takashi Shinohara

Biology of reproduction, 104(3) 706-716, Mar 11, 2021
An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia.

Hiroko Morimoto, Takuya Yamamoto, Takehiro Miyazaki, Narumi Ogonuki, Atsuo Ogura, Takashi Tanaka, Mito Kanatsu-Shinohara, Chihiro Yabe-Nishimura, Hongliang Zhang, Yves Pommier …

Genes & development, 35(3-4) 250-260, Feb 1, 2021
CD2 is a surface marker for mouse and rat spermatogonial stem cells.

Mito Kanatsu-Shinohara, Guiying Chen, Hiroko Morimoto, Takashi Shinohara

The Journal of reproduction and development, 66(4) 341-349, Aug 20, 2020 Peer-reviewed
Autologous transplantation of spermatogonial stem cells restores fertility in congenitally infertile mice.

Mito Kanatsu-Shinohara, Narumi Ogonuki, Shogo Matoba, Atsuo Ogura, Takashi Shinohara

Proceedings of the National Academy of Sciences of the United States of America, 117(14) 7837-7844, Apr 7, 2020 Peer-reviewed
Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein.

Takashi Shinohara, Mito Kanatsu-Shinohara

Stem cell reports, 14(3) 447-461, Mar 10, 2020 Peer-reviewed
Aging of spermatogonial stem cells by Jnk-mediated glycolysis activation.

Mito Kanatsu-Shinohara, Takuya Yamamoto, Hidehiro Toh, Yasuhiro Kazuki, Kanako Kazuki, Junichi Imoto, Kazuho Ikeo, Motohiko Oshima, Katsuhiko Shirahige, Atsushi Iwama …

Proceedings of the National Academy of Sciences of the United States of America, 116(33) 16404-16409, Aug 13, 2019 Peer-reviewed
ROS amplification drives mouse spermatogonial stem cell self-renewal.

Hiroko Morimoto, Mito Kanastu-Shinohara, Narumi Ogonuki, Satoshi Kamimura, Atsuo Ogura, Chihiro Yabe-Nishimura, Yoshifumi Mori, Takeshi Morimoto, Satoshi Watanabe, Kinya Otsu …

Life science alliance, 2(2), Apr, 2019 Peer-reviewed
Sendai virus-mediated transduction of mammalian spermatogonial stem cells.

Watanabe, S, Kanatsu-Shinohara, M, Shinohara, T

Biol. Reprod., 100 523-534, 2019 Peer-reviewed
Culture and transplantation of spermatogonial stem cells.

Takashima, S, Shinohara, T

Stem Cell Res., 29 46-55, 2018 Peer-reviewed
In vivo genetic manipulation of male germline stem cells and their microenvironment by adeno-associated viruses.

Watanabe, S, Kanatsu-Shinohara, M, Ogonuki, N, Matoba, S, Ogura, A, Shinohara, T

Stem Cell Reports, 10 1551-1564, 2018 Peer-reviewed

Misc.

精子幹細胞の機能的寿命の維持機構

篠原美都, 本田直樹, 篠原隆司

実験医学, 35(8) 1297-1302, May, 2017 Invited
Aging of Male Germline Stem Cells

Anti-aging medicine, 9(1) 30-34, Feb, 2013
Transcriptional and epigenetic landscape of mouse germline stem cells

Mihoko Hosokawa, Ami Katanaya, Eri Hayashi, Ayako Mochizuki, Hirofami Suemori, Koji Numata, Kuniya Abe, Takashi Tanaka, Mito Kanatsu-Shinohara, Takashi Shinohara …

GENES & GENETIC SYSTEMS, 87(6) 388-388, Dec, 2012
マウス生殖幹細胞のゲノムーエピゲノム解析

細川美穂子, 刀谷在美, 林瑛理, 望月綾子, 末盛博文, 沼田興治, 阿部訓也, 田中敬, 篠原美都, 篠原隆司 …

日本遺伝学会第84 回大会（2012.9.26，福岡）, 2012 Peer-reviewed
マウス生殖幹細胞の体細胞型増殖から第一減数分裂前期への移行

細川美穂子, 田中敬, 林瑛理, 篠原美都, 篠原隆司, 中辻憲夫, 中馬新一郎

特定領域研究生殖サイクル第4 回公開シンポジウム（11.17-18, 2011, 大阪）, 2011 Peer-reviewed
Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation

Mito Kanatsu-Shinohara, Seiji Takashima, Takashi Shinohara

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(14) 6210-6215, Apr, 2010 Peer-reviewed
マウス生殖幹細胞の減数分裂移行実験系作出の試み

細川美穂子, 田中敬, 林瑛理, 篠原美都, 篠原隆司, 中辻憲夫, 中馬新一郎

特定領域研究「生殖サイクル若手勉強会2010」（2010. 7. 21－23. Tsukuba）, 2010 Peer-reviewed
Production of Transgenic Rats via Lentiviral Transduction and Xenogeneic Transplantation of Spermatogonial Stem Cells Editorial Comment

M. Kanatsu-Shinohara, M. Kato, M. Takehashi, H. Morimoto, S. Takashima, S. Chuma, N. Nakatsuji, M. Hirabayashi, T. Shinohara

JOURNAL OF UROLOGY, 182(1) 392-392, Jul, 2009
Pluripotency of spermatogonial stem cells and their potential in medical applications

64 581-587, Mar, 2009
HERITABLE IMPRINTING DEFECT CAUSED BY FETAL MALE GERM CELL CULTURE IN MICE

Takashi Shinohara, Mito Kanatsu-Shinohara, Jiyoung Lee

JOURNAL OF PHYSIOLOGICAL SCIENCES, 59 71-71, 2009
精子幹細胞と再生医療

竹橋正則, 篠原美都, 篠原隆司

産科と婦人科, 76(10) 1250-1254, 2009
Germline modification in spermatogonial stem cells

Takashi Shinohara

BIOLOGY OF REPRODUCTION, 120-120, 2008
精子幹細胞による新しい遺伝子改変ストラテジー

竹橋正則, 篠原美都, 篠原隆司

実験医学, 26(5) 649-654, 2008
トピックス：精子幹細胞の培養と遺伝子改変

竹橋正則, 篠原美都, 篠原隆司

最新医学, 63(9) 1768-1773, 2008
Spermatogonial stem cells: utility in the production of gene knockout and their unexpected features

Regenerative medicine, 6(3) 258-262, Aug, 2007
再生医学のいま--基礎研究から臨床への展開に向けて(9)精子幹細胞研究を用いたノックアウト動物の作成とその意外な展開

篠原隆司, 篠原美都

治療, 89(7) 2354-2359, Jul, 2007
Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells

Arata Honda, Michiko Hirose, Kenshiro Hara, Shogo Matoba, Kimiko Inoue, Himomi Miki, Hitoshi Hiura, Mito Kanatsu-Shinohara, Yoshiakira Kanai, Tomohiro Kono …

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 104(30) 12389-12394, Jul, 2007 Peer-reviewed
Adenovirus-mediated gene delivery into mouse spermatogonial stem cells

Masanori Takehashi, Mito Kanatsu-Shinohara, Kimiko Inoue, Narumi Ogonuki, Hiromi Miki, Shinya Toyokuni, Atsuo Ogura, Takashi Shinohara

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 104(8) 2596-2601, Feb, 2007 Peer-reviewed
Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination

Takashi Shinohara, Megumi Kato, Masanori Takehashi, Jiyoung Lee, Shinichiro Chuma, Norio Nakatsuji, Mito Kanatsu-Shinohara, Masumi Hirabayashi

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103(37) 13624-13628, Sep, 2006 Peer-reviewed
Functional Restoration of a Genetic Defect in Multipotent Germline Stem Cells by Human Artificial Chromosome Containing a Genomic Insert

Yasuhiro Kazuki, Hidetoshi Hoshiya, Yoshiteru Kai, Satoshi Abe, Masato Takiguchi, Mitsuhiko Osaki, Mito Kanatsu-Shinohara, Naoyo Kajitani, Yasuaki Shirayoshi, Masaharu Hiratuka …

MOLECULAR THERAPY, 13 S403-S404, May, 2006

Research Projects

Next-generation ART: Building a global network to create innovative technologies in m ammalian reproductive engineering

Grants-in-Aid for Scientific Research, Fund for the Promotion of Joint International Research (International Leading Research ), Japan Society for the Promotion of Science, Nov, 2023 - Mar, 2030
Isolation and functional analysis of genes controlling totipotency

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Jun, 2019 - Mar, 2024
Grant-in-Aid for Scientific Research on Innovative Areas "Totipotency" steering group

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Jun, 2019 - Mar, 2024
Molecular Analysis of Spermatogonial Stem Cell Aging

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), Japan Society for the Promotion of Science, Jun, 2018 - Mar, 2023
Functional analysis and culture of human spermatogonial stem cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Japan Society for the Promotion of Science, Jun, 2019 - Mar, 2022

Shinohara Takashi
Behavior analysis of offspring derived from spermatogonial stem cell transplantation

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2021
Functional analysis of suppressive histone modification in sex determination

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2020
epigenome dynamics and regulation in germ cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2019
Support for international research in "epigenome dynamics and regulation in germ cells"

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Nov, 2015 - Mar, 2018

Takashi Shinohara
Genetic engineering of primordial germ cells for germline modification

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018

Shinohara Takashi, MORIMOTO Hiroko
Analysis of epigenome stability and germ cell tumor formation

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Jun, 2013 - Mar, 2018

Shinohara Takashi
Regulation of epigenome dynamics and regulation in germ cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Jun, 2013 - Mar, 2018

Shinohara Takashi, SAGA Yumiko, CHUMA Shinichiro, SAITO Kuniaki, NAKAMURA Toshinobu, TSUKADA Yuichi, OGURA Atsuo, KOHDA Takashi, SASAKI Hiroyuki
Analyses and regulation of germline epigenome

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Jun, 2013 - Mar, 2018
The role of ROS in spermatogonial stem cell self-renewal

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2013 - Mar, 2016

SHINOHARA Takashi
Reciprocal conversion between germ cells and pluripotent stem cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

KIMURA Tohru, SHINOHARA Takashi, IKAWA Masahito, NADA Shigeyuki
Development of a long-term culture system for functional Sertoli cells.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, 2011 - 2013

SHINOHARA Takashi
Analyses on the quality control mechanism of mouse spermatogonial stem cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas, Japan Society for the Promotion of Science, 2008 - 2012

SHINOHARA Takashi
精子形成の試験管内誘導

科学研究費助成事業, 挑戦的萌芽研究, 日本学術振興会, 2008 - 2010

篠原隆司
Production of knockout animals by spermatogonial stem cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, 2007 - 2010

SHINOHARA Takashi
The improvement of microinsemination techniques and the production of transgenic offspring with GS cell-derived spermatids in the rats

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2007 - 2009

HIRABAYASHI Masumi, SHINOHARA Takashi