論文

国際誌
2022年4月12日

Regeneration of spermatogenesis by mouse germ cell transplantation into allogeneic and xenogeneic testis primordia or organoids.

Stem cell reports
  • Mito Kanatsu-Shinohara
  • ,
  • Narumi Ogonuki
  • ,
  • Shogo Matoba
  • ,
  • Hiroko Morimoto
  • ,
  • Yusuke Shiromoto
  • ,
  • Atsuo Ogura
  • ,
  • Takashi Shinohara

17
4
開始ページ
924
終了ページ
935
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.stemcr.2022.02.013

Gametogenesis requires close interactions between germ cells and somatic cells. Derivation of sperm from spermatogonial stem cells (SSCs) is hampered by the inefficiency of spermatogonial transplantation technique in many animal species because it requires a large number of SSCs and depletion of endogenous spermatogenesis. Here we used mouse testis primordia and organoids to induce spermatogenesis from SSCs. We microinjected mouse SSCs into embryonic gonads or reaggregated neonatal testis organoids, which were transplanted under the tunica albuginea of mature testes. As few as 1 × 104 donor cells colonized both types of transplants and produced sperm. Moreover, rat embryonic gonads supported xenogeneic spermatogenesis from mouse SSCs when transplanted in testes of immunodeficient mice. Offspring with normal genomic imprinting patterns were born after microinsemination. These results demonstrate remarkable flexibility of the germ cell-somatic cell interaction and raise new strategies of SSC manipulation for animal transgenesis and analysis of male infertility.

リンク情報
DOI
https://doi.org/10.1016/j.stemcr.2022.02.013
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35334214
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023780
ID情報
  • DOI : 10.1016/j.stemcr.2022.02.013
  • PubMed ID : 35334214
  • PubMed Central 記事ID : PMC9023780

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