1991年7月
PREPARATION OF SOLUBLE MURINE IL-6 RECEPTOR AND ANTIMURINE IL-6 RECEPTOR ANTIBODIES
JOURNAL OF IMMUNOLOGY
- 巻
- 147
- 号
- 1
- 開始ページ
- 168
- 終了ページ
- 173
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- AMER ASSOC IMMUNOLOGISTS
Starting with a previously isolated cDNA clone encoding murine IL-6R, a stable transformed Chinese hamster ovary cell line constitutively expressing soluble murine IL-6R (smIL-6R) has been established. The smIL-6R was purified to homogeneity by sequential filtration and chromatography of culture medium. The smIL-6R augmented the sensitivity of M1 cells to IL-6 in their growth inhibition in a dose-response manner. Rat hybridomas producing mAb specific to murine IL-6R were also established. One of the clones, RS13, produced IgG2a isotype that was capable of inhibiting IL-6 activity. ELISA for the quantitation of smIL-6R was established, which could detect smlL-6R in a quantity as low as 1 ng/ml.
- リンク情報
- ID情報
-
- ISSN : 0022-1767
- Web of Science ID : WOS:A1991FT76300025