1994年3月
NITRIC-OXIDE BLOCKS THE CELL-CYCLE OF MOUSE MACROPHAGE-LIKE CELLS IN THE EARLY G(2)+M PHASE
FEBS LETTERS
- ,
- ,
- ,
- ,
- 巻
- 340
- 号
- 3
- 開始ページ
- 159
- 終了ページ
- 162
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/0014-5793(94)80128-2
- 出版者・発行元
- ELSEVIER SCIENCE BV
The effects of nitric oxide produced by macrophage-like cells (Mm1) on the cell cycle were investigated. Mm1 cells lost proliferative activity in the presence of interleukin-6 (IL-6) and a subpopulation accumulated in the G(2)+M phase. This level increased in proportion to the incubation lime. The DNA content of the cells was slightly lower than that of Mm1 cells treated with vinbrastine or demecolcine, drugs which block the cell cycle in the M phase. The peak of the early G(2)+M phase was reduced by treatment with N-G-mono-methyl-L-arginifie. However, after treatment with exogenous nitric oxide or sodium nitroprusside, the G(0)/G(1) phase increased, but the early-G(2)+M and the S phase decreased. The flow cytometry pattern in IL-6-treated Mm1 was the same as that of cytochalasin B-treated Mm1. These data suggest that endogenous nitric oxide affects the microfilament system of IL-6-treated Mm1 cells and blocks the cell cycle in the early G(2)+M phase.
- リンク情報
- ID情報
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- DOI : 10.1016/0014-5793(94)80128-2
- ISSN : 0014-5793
- Web of Science ID : WOS:A1994NB03700002