2007年8月
Improvement in performance of affinity gels containing gly-D-phe as a ligand to thermolysin due to increasing the Spacer chain length
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- ,
- ,
- ,
- 巻
- 71
- 号
- 8
- 開始ページ
- 2083
- 終了ページ
- 2086
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.70178
- 出版者・発行元
- TAYLOR & FRANCIS LTD
The aim of this stud), was to improve the performance of affinity gels containing glycyl-D-phenylalanine (Gly-D-Phe) as a ligand to thermolysin. Gly-D-Phe was immobilized to the resin through spacers of varying chain lengths. The resulting affinity gels had spacer chain lengths of 2 carbon atoms and 11 and 13 carbon-and-oxygen atoms (designated T2, T11, and T13), and were characterized for their binding abilities to thermolysin. Measurement of adsorption isotherms showed that the association constants to thermolysin were in the order T13 > T11 > T2. In affinity column chromatography, in which 5 mg thermolysin was applied onto 1-ml volumes of the gels, the adsorption ratios of thermolysin were also in the order T13 > T11 > T2. These results indicate that the performance of affinity gels is improved by increasing the spacer chain length to 13 carbon-and-oxygen atoms.
- リンク情報
-
- DOI
- https://doi.org/10.1271/bbb.70178
- CiNii Articles
- http://ci.nii.ac.jp/naid/10027518177
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17690447
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000249194600040&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1271/bbb.70178
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 10027518177
- PubMed ID : 17690447
- Web of Science ID : WOS:000249194600040