2011年4月
Chemiluminescent Enzyme Immunoassay for Measuring Leptin
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- ,
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- 巻
- 75
- 号
- 4
- 開始ページ
- 752
- 終了ページ
- 756
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.100885
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Leptin is one of the representative adipocyte-derived protein hormones. Measuring the serum leptin concentration gives an important index for preventing and treating diabetes mellitus and other diseases. We constructed in this study a chemiluminescent enzyme immunoassay (CLEIA) for measuring leptin by using the anti-leptin polyclonal antibody and alkaline phosphatase (ALP). The method applies the IgG-conjugated ferrite particle to capture leptin in a sample and the ALP-conjugated Fab fragment to detect the captured leptin. We tested Block ace, CE510, and bovine serum albumin (BSA) for their abilities to block non-specific binding of ALP-conjugated anti-leptin Fab to the ferrite particle and found BSA to be the most effective. The measurable range with this ELISA for leptin was 0.1-1.0 pg/mL of leptin and the detection limit (blank + 2SD) was 0.1 pg/mL of leptin. These results demonstrate sufficient sensitivity with our system to measure the serum leptin concentration and its clinical usefulness. The results also suggest that a sensitive enzyme immunoassay can be constructed by using only one polyclonal antibody.
- リンク情報
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- DOI
- https://doi.org/10.1271/bbb.100885
- CiNii Articles
- http://ci.nii.ac.jp/naid/10028271790
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/21512229
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000290927000023&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1271/bbb.100885
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 10028271790
- PubMed ID : 21512229
- Web of Science ID : WOS:000290927000023