2012年11月
Establishment of a set of inbred strains of the pine wood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), and evidence of their varying levels of virulence
APPLIED ENTOMOLOGY AND ZOOLOGY
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- 巻
- 47
- 号
- 4
- 開始ページ
- 341
- 終了ページ
- 350
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s13355-012-0124-8
- 出版者・発行元
- SPRINGER JAPAN KK
Pine wilt disease (PWD) caused by the pine wood nematode, Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle, has become a worldwide problem. The pathogenic mechanism of PWD continues to remain controversial, which in part may be attributed to the lack of universal materials of B. xylophilus with a high genetic purity. The intrinsic high genetic diversity in B. xylophilus isolates/populations must be a fatal obstacle for performing forward genetics and other molecular approaches to controlling them. We conducted a series of successive full-sib mating of conventional isolates of B. xylophilus to establish a set of inbred strains. Using DNA markers, we also determined their genetic diversity and biological characteristics, such as virulence and reproductive ability. Consequently, the newly established strains yielded a higher genetic purity than the conventional isolates and showed varying virulence despite sharing a common ancestor. The significance of this study lies not only in establishing a set of inbred strains of B. xylophilus with the certification of their purity but also in demonstrating that avirulent strain(s) with a genotype similar to the virulent strains can be obtained by simple successive full-sib mating. This technique is one of the most powerful tools for elucidating the pathogenic mechanism(s) of PWD.
- リンク情報
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- DOI
- https://doi.org/10.1007/s13355-012-0124-8
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201202214238863900
- CiNii Articles
- http://ci.nii.ac.jp/naid/40019495753
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000310610400005&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1007/s13355-012-0124-8
- ISSN : 0003-6862
- J-Global ID : 201202214238863900
- CiNii Articles ID : 40019495753
- Web of Science ID : WOS:000310610400005