2012年12月
Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells.
Nature communications
- 巻
- 3
- 号
- 1
- 開始ページ
- 1236
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/ncomms2231
- 出版者・発行元
- Nature Publishing Group
細胞接着タンパクを用い、 安全・高効率なヒトES / iPS細胞の培養法を開発 : 幹細胞実用化に必要な品質保証・大量生産に向けて前進. 京都大学プレスリリース. 2012-12-05.Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications.
- リンク情報
- ID情報
-
- DOI : 10.1038/ncomms2231
- ISSN : 2041-1723
- eISSN : 2041-1723
- CiNii Articles ID : 120005025430
- PubMed ID : 23212365