MISC

査読有り
2008年9月

Generation of functional erythrocytes from human embryonic stem cell-derived definitive hematopoiesis

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Feng Ma
  • Yasuhiro Ebihara
  • Katsutsugu Umeda
  • Hiromi Sakai
  • Sachiyo Hanada
  • Hong Zhang
  • Yuji Zaike
  • Eishun Tsuchida
  • Tatsutoshi Nakahata
  • Hiromitsu Nakauchi
  • Kohichiro Tsuji
  • 全て表示

105
35
開始ページ
13087
終了ページ
13092
記述言語
英語
掲載種別
DOI
10.1073/pnas.0802220105
出版者・発行元
NATL ACAD SCIENCES

A critical issue for clinical utilization of human ES cells (hESCs) is whether they can generate terminally mature progenies with normal function. We recently developed a method for efficient production of hematopoietic progenitors from hESCs by coculture with murine fetal liver-derived stromal cells. Large numbers of hESCs-derived erythroid progenitors generated by the coculture enabled us to analyze the development of erythropoiesis at a clone level and investigate their function. The results showed that the globin expression in the erythroid cells in individual clones changed in a time-dependent manner. In particular, embryonic epsilon-globin-expressing erythroid cells from individual clones decreased, whereas adult-type beta-globin-expressing cells increased to approximate to 100% in all clones we examined, indicating that the cells undergo definitive hematopoiesis. Enucleated erythrocytes also appeared among the clonal progeny. A comparison analysis showed that hESC-derived erythroid cells took a similar differentiation pathway to human cord blood CD34(+) progenitor-derived cells when examined for the expression of glycophorin A, CD71 and CD81. Furthermore, these hESC-derived erythroid cells could function as oxygen carriers and had a sufficient glucose-6-phosphate dehydrogenase activity. The present study should provide an experimental model for exploring early development of human erythropoiesis and hemoglobin switching and may help in the discovery of drugs for hereditary diseases in erythrocyte development.

リンク情報
DOI
https://doi.org/10.1073/pnas.0802220105
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000259343000083&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.0802220105
  • ISSN : 0027-8424
  • Web of Science ID : WOS:000259343000083

エクスポート
BibTeX RIS