2003年12月
Regiospecific analysis by ethanolysis of oil with immobilized Candida antarctica lipase
Lipids
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- 巻
- 38
- 号
- 12
- 開始ページ
- 1281
- 終了ページ
- 1286
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s11745-003-1190-x
- 出版者・発行元
- AMER OIL CHEMISTS SOC A O C S PRESS
A mixture of oil/ethanol (1:3, w/w) was shaken at 30degreesC with 4% immobilized Candida antarctica lipase by weight of the reaction mixture. The reaction regiospecifically converted FA at the 1- and 3-positions to FA ethyl esters, and the lipase acted on C-14-C-24 FA to a similar degree. The content of 2-MAG reached a maximum after 4 h; the content was 28-29 mol% based on the total amount of FA in the reaction mixture at 59-69% ethanolysis. Only 2-MAG were present in the reaction mixture during the first 4 h, and 1(3)-MAG were detected after 7 h. After removal of ethanol from the 4-h reaction mixture by evaporation, 2-MAG were fractionated by silica gel column chromatography. The contents of FA in the 2-MAG obtained by ethanolysis of several oils coincided well with FA compositions at the 2-position, which was analyzed by Grignard degradation. It was shown that ethanolysis of oil with C. antarctica lipase can be applied to analysis of FA composition at the 2-position in TAG.
- リンク情報
- ID情報
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- DOI : 10.1007/s11745-003-1190-x
- ISSN : 0024-4201
- Web of Science ID : WOS:000188323300010