論文

査読有り
2014年12月

Quantitative analysis of motifs contributing to the interaction between PLS-subfamily members and their target RNA sequences in plastid RNA editing

PLANT JOURNAL
  • Kenji Okuda
  • ,
  • Harumi Shoki
  • ,
  • Miho Arai
  • ,
  • Toshiharu Shikanai
  • ,
  • Ian Small
  • ,
  • Takahiro Nakamura

80
5
開始ページ
870
終了ページ
882
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/tpj.12687
出版者・発行元
WILEY-BLACKWELL

In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. Target cytidines are specifically recognized by pentatricopeptide repeat (PPR) proteins of the PLS subfamily, which have additional C-terminal E or E-DYW motifs. Recent insilico analysis proposed a model for site recognition by PLS-subfamily PPR proteins, with a correspondence of one PPR motif to one nucleotide, and with the C-terminal last Smotif aligning with the nucleotide at position -4 with respect to the editing site. Here, we present quantitative biochemical data on site recognition by four PLS-subfamily proteins: CRR28 and OTP85 are DYW-class members, whereas CRR21 and OTP80 are E-class members. The minimal RNA segments required for high-affinity binding by these PPR proteins were experimentally determined. The results were generally consistent with the insilico-based model; however, we clarified that several PPR motifs, including the C-terminal L2 and Smotifs of CRR21 and OTP80, are dispensable for the RNA binding, suggesting distinct contributions of each PPR motif to site recognition. We also demonstrate that the DYW motif interacts with the target C and its 5 proximal region (from -3 to 0), whereas the Emotif is not involved in binding.

リンク情報
DOI
https://doi.org/10.1111/tpj.12687
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25279799
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000345511500011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/tpj.12687
  • ISSN : 0960-7412
  • eISSN : 1365-313X
  • PubMed ID : 25279799
  • Web of Science ID : WOS:000345511500011

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