2004年7月
Ribosomal RNA processing and an RNase R family member in chloroplasts of Arabidopsis
PLANT MOLECULAR BIOLOGY
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- 巻
- 55
- 号
- 4
- 開始ページ
- 595
- 終了ページ
- 606
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s11103-004-1507-1
- 出版者・発行元
- KLUWER ACADEMIC PUBL
An Arabidopsis mutant rnr1, which has a defect in the basic genetic system in chloroplasts, was isolated using the screening of the high chlorophyll fluorescence phenotype. Whereas chlorophyll fluorescence and immunoblot studies showed the mutant had reduced activities of photosystems I and II, molecular characterization of the mutant suggested that a T-DNA insertion impaired the expression of a gene encoding a RNase R family member with a targeting signal to chloroplasts. Since RNase R family members have a 3'-5'exoribonuclease activity, we examined the RNA profile in chloroplasts. In rnr1 the intercistronic cleavage between 23S and 4.5S rRNA was impaired, and a significant reduction in rRNA in chloroplasts was found, suggesting that RNR1 functions in the maturation of chloroplast rRNA. The present results suggest that defects in the genetic system in chloroplasts cause high chlorophyll fluorescence, pale green leaf, and marked reduction in the growth rate, whereas the levels of some chloroplast RNA were higher in rnr1 than in the wild-type.
- リンク情報
- ID情報
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- DOI : 10.1007/s11103-004-1507-1
- ISSN : 0167-4412
- PubMed ID : 15604703
- Web of Science ID : WOS:000225690300011