2010年7月
Dual retrovirus integration tagging: identification of new signaling molecules Fiz1 and Hipk2 that are involved in the IL-7 signaling pathway in B lymphoblastic lymphomas
JOURNAL OF LEUKOCYTE BIOLOGY
- 巻
- 88
- 号
- 1
- 開始ページ
- 107
- 終了ページ
- 116
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1189/jlb.1109748
- 出版者・発行元
- FEDERATION AMER SOC EXP BIOL
IL-7R, FLT3, and CD43 are surface antigens expressed during the transition from pro-B to pre-B cells in BM. To understand interactions between their signaling pathways, we analyzed spontaneous mouse B-LBLs with dual MLV integration into Stat5a and Fiz1 or Stat5a and Hipk2. MLV integration resulted in up-regulation of these genes in lymphoma cells compared with normal pro-B cells from the BM. In lymphomas with both integrations into Stat5a and Fiz1, increases in phosphorylated STAT5A and expression of c-Myc, a target gene of STAT5A, were observed following stimulation of the FLT3. Clones with the dual integrations grew faster in IL-7 and FLT3L-supplemented medium than clones with Stat5a integration alone. On the other hand, in lymphomas with integrations into Stat5a and Hipk2, increases in phosphorylated STAT5A and expression of c-Myc were observed following cross- linking of CD43. In conclusion, FLT3 and CD43 signaling pathways involve STAT5A via Fiz1 and Hipk2 in B-LBLs. Identification of the dual MLV integration sites in B-LBLs, therefore, will provide an excellent tool for identification of the signaling pathways in B-LBLs. J. Leukoc. Biol. 88: 107-116; 2010.
- リンク情報
-
- DOI
- https://doi.org/10.1189/jlb.1109748
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201102274918025319
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20360400
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000279356700012&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1189/jlb.1109748
- ISSN : 0741-5400
- J-Global ID : 201102274918025319
- PubMed ID : 20360400
- Web of Science ID : WOS:000279356700012