論文

査読有り 最終著者 責任著者
2017年5月

Holliday junction resolvases mediate chloroplast nucleoid segregation

SCIENCE
  • Yusuke Kobayashi
  • Osami Misumi
  • Masaki Odahara
  • Kota Ishibashi
  • Masafumi Hirono
  • Kumi Hidaka
  • Masayuki Endo
  • Hiroshi Sugiyama
  • Hiroshi Iwasaki
  • Tsuneyoshi Kuroiwa
  • Toshiharu Shikanai
  • Yoshiki Nishimura
  • 全て表示

356
6338
開始ページ
631
終了ページ
634
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1126/science.aan0038
出版者・発行元
AMER ASSOC ADVANCEMENT SCIENCE

Holliday junctions, four-stranded DNA structures formed during homologous recombination, are disentangled by resolvases that have been found in prokaryotes and eukaryotes but not in plant organelles. Here, we identify monokaryotic chloroplast 1 (MOC1) as a Holliday junction resolvase in chloroplasts by analyzing a green alga Chlamydomonas reinhardtii mutant defective in chloroplast nucleoid (DNA-protein complex) segregation. MOC1 is structurally similar to a bacterial Holliday junction resolvase, resistance to ultraviolet (Ruv) C, and genetically conserved among green plants. Reduced or no expression of MOC1 in Arabidopsis thaliana leads to growth defects and aberrant chloroplast nucleoid segregation. In vitro biochemical analysis and high-speed atomic force microscopic analysis revealed that A. thaliana MOC 1 (AtMOC1) binds and cleaves the core of Holliday junctions symmetrically. MOC1 may mediate chloroplast nucleoid segregation in green plants by resolving Holliday junctions.

リンク情報
DOI
https://doi.org/10.1126/science.aan0038
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000400975200053&DestApp=WOS_CPL
URL
http://orcid.org/0000-0001-8686-9206
ID情報
  • DOI : 10.1126/science.aan0038
  • ISSN : 0036-8075
  • eISSN : 1095-9203
  • ORCIDのPut Code : 34127233
  • Web of Science ID : WOS:000400975200053

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