2015年2月
Live-cell Imaging of Endogenous mRNAs with a Small Molecule
Angewandte Chemie International Edition in English
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- 巻
- 127
- 号
- 6
- 開始ページ
- 1875
- 終了ページ
- 1878
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/anie.201410339
- 出版者・発行元
- WILEY-V C H VERLAG GMBH
Determination of subcellular localization and dynamics of mRNA is increasingly important to understanding gene expression. A new convenient and versatile method is reported that permits spatiotemporal imaging of specific non-engineered RNAs in living cells. The method uses transfection of a plasmid encoding a gene-specific RNA aptamer, combined with a cell-permeable synthetic small molecule, the fluorescence of which is restored only when the RNA aptamer hybridizes with its cognitive mRNA. The method was validated by live-cell imaging of the endogenous mRNA of -actin. Application of the technology to mRNAs of a total of 84 human cytoskeletal genes allowed us to observe cellular dynamics of several endogenous mRNAs including arfaptin-2, cortactin, and cytoplasmic FMR1-interacting protein2. The RNA-imaging technology and its further optimization might permit live-cell imaging of any RNA molecules.
- リンク情報
- ID情報
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- DOI : 10.1002/anie.201410339
- ISSN : 1433-7851
- eISSN : 1521-3773
- Web of Science ID : WOS:000349209200031