2016年4月
Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
- 巻
- 72
- 号
- 開始ページ
- 520
- 終了ページ
- 523
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1107/S2059798316001480
- 出版者・発行元
- INT UNION CRYSTALLOGRAPHY
A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 mm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 angstrom from < 0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.
- リンク情報
- ID情報
-
- DOI : 10.1107/S2059798316001480
- ISSN : 2059-7983
- Web of Science ID : WOS:000373718500007